Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation

Elana Ben-Akiva, Kelly R. Rhodes, Randall A. Meyer, Jordan Green

Research output: Contribution to journalArticle

Abstract

Artificial antigen presenting cells (aAPC) are a promising platform for immune modulation due to their potent ability to stimulate T cells. Acellular substrates offer key advantages over cell-based aAPC, including precise control of signal presentation parameters and physical properties of the aAPC surface to modulate its interactions with T cells. aAPC constructed from anisotropic particles, particularly ellipsoidal particles, have been shown to be more effective than their spherical counterparts at stimulating T cells due to increased binding and larger surface area available for T cell contact, as well as reduced nonspecific uptake and enhanced pharmacokinetic properties. Despite increased interest in anisotropic particles, even widely accepted methods of generating anisotropic particles such as thin-film stretching can be challenging to implement and use reproducibly. To this end, we describe a protocol for the rapid, standardized fabrication of biodegradable anisotropic particle-based aAPC with tunable size, shape, and signal presentation for T cell expansion ex vivo or in vivo, along with methods to characterize their size, morphology, and surface protein content, and to assess their functionality. This approach to fabricating anisotropic aAPC is scalable and reproducible, making it ideal for generating aAPC for "off-the-shelf" immunotherapies.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number140
DOIs
StatePublished - Oct 12 2018

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T-cells
Antigen-Presenting Cells
Chemical activation
T-Lymphocytes
Fabrication
Artificial Cells
Pharmacokinetics
Immunotherapy
Stretching
Membrane Proteins
Physical properties
Modulation
Proteins
Thin films
Substrates

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Fabrication of Anisotropic Polymeric Artificial Antigen Presenting Cells for CD8+ T Cell Activation. / Ben-Akiva, Elana; Rhodes, Kelly R.; Meyer, Randall A.; Green, Jordan.

In: Journal of visualized experiments : JoVE, No. 140, 12.10.2018.

Research output: Contribution to journalArticle

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