EzrA contributes to the regulation of cell size in Staphylococcus aureus

Ana M. Jorge, Egbert Hoiczyk, João P. Gomes, Mariana G. Pinho

Research output: Contribution to journalArticle

Abstract

EzrA is a negative regulator of FtsZ in Bacillus subtilis, involved in the coordination between cell growth and cell division and in the control of the cell elongation-division cycle. We have now studied the role of the Staphylococcus aureus homologue of the B. subtilis EzrA protein and shown that it is not essential for cell viability. EzrA conditional and null mutants have an overall increase of the average cell size, compared to wild type strains. In the larger ezrA mutant S. aureus cells, cell division protein FtsZ and the cell wall synthesizing Penicillin Binding Proteins (PBPs) are not properly localized. This suggests that there may be a maximum cell diameter that allows formation of a Z-ring capable of recruiting the other components of the divisome and of driving cytokinesis. We propose that the major role of EzrA in S. aureus is in cell size homeostasis.

Original languageEnglish (US)
Article numbere27542
JournalPLoS One
Volume6
Issue number11
DOIs
StatePublished - Nov 14 2011

Fingerprint

Cell Size
Staphylococcus aureus
Cells
Cell Division
Bacillus subtilis
Penicillin-Binding Proteins
cell division
cell growth
Cytokinesis
cells
mutants
Cell Wall
Cell Survival
Cell Cycle
cytokinesis
Cell growth
Homeostasis
Bacilli
penicillins
cell viability

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Jorge, A. M., Hoiczyk, E., Gomes, J. P., & Pinho, M. G. (2011). EzrA contributes to the regulation of cell size in Staphylococcus aureus. PLoS One, 6(11), [e27542]. https://doi.org/10.1371/journal.pone.0027542

EzrA contributes to the regulation of cell size in Staphylococcus aureus. / Jorge, Ana M.; Hoiczyk, Egbert; Gomes, João P.; Pinho, Mariana G.

In: PLoS One, Vol. 6, No. 11, e27542, 14.11.2011.

Research output: Contribution to journalArticle

Jorge, Ana M. ; Hoiczyk, Egbert ; Gomes, João P. ; Pinho, Mariana G. / EzrA contributes to the regulation of cell size in Staphylococcus aureus. In: PLoS One. 2011 ; Vol. 6, No. 11.
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