Extremely high levels of NADPH in guinea pig lens: Correlation with zeta-crystallin concentration

P. Vasantha Rao, J. Samuel Zigler

Research output: Contribution to journalArticle

Abstract

Zeta-crystallin, a major "taxon-specific" protein of the guinea pig lens, specifically binds NADPH. Analysis of pyridine nucleotide levels in guinea pig lens revealed values for NADPH approximately 50-fold higher than in other lenses. Indeed to our knowledge the values reported are higher than have been observed in any tissue. A clear correlation exists between NADPH and zeta-crystallin contents of the lens both in normal guinea pigs during development and in a line of guinea pigs with a mutation in the gene for zeta-crystallin. Heterozygotes for this mutation had a 50% reduction in NADPH, while homozygotes have only about 6% of the normal level. NADP+ levels were also markedly elevated suggesting that redox cycling of the NADPH is occurring.

Original languageEnglish (US)
Pages (from-to)1221-1228
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number3
DOIs
StatePublished - Mar 30 1990

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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