TY - JOUR
T1 - Extremely high levels of NADPH in guinea pig lens
T2 - Correlation with zeta-crystallin concentration
AU - Rao, P. Vasantha
AU - Zigler, J. Samuel
PY - 1990/3/30
Y1 - 1990/3/30
N2 - Zeta-crystallin, a major "taxon-specific" protein of the guinea pig lens, specifically binds NADPH. Analysis of pyridine nucleotide levels in guinea pig lens revealed values for NADPH approximately 50-fold higher than in other lenses. Indeed to our knowledge the values reported are higher than have been observed in any tissue. A clear correlation exists between NADPH and zeta-crystallin contents of the lens both in normal guinea pigs during development and in a line of guinea pigs with a mutation in the gene for zeta-crystallin. Heterozygotes for this mutation had a 50% reduction in NADPH, while homozygotes have only about 6% of the normal level. NADP+ levels were also markedly elevated suggesting that redox cycling of the NADPH is occurring.
AB - Zeta-crystallin, a major "taxon-specific" protein of the guinea pig lens, specifically binds NADPH. Analysis of pyridine nucleotide levels in guinea pig lens revealed values for NADPH approximately 50-fold higher than in other lenses. Indeed to our knowledge the values reported are higher than have been observed in any tissue. A clear correlation exists between NADPH and zeta-crystallin contents of the lens both in normal guinea pigs during development and in a line of guinea pigs with a mutation in the gene for zeta-crystallin. Heterozygotes for this mutation had a 50% reduction in NADPH, while homozygotes have only about 6% of the normal level. NADP+ levels were also markedly elevated suggesting that redox cycling of the NADPH is occurring.
UR - http://www.scopus.com/inward/record.url?scp=0025275453&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025275453&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)90654-6
DO - 10.1016/0006-291X(90)90654-6
M3 - Article
C2 - 2322267
AN - SCOPUS:0025275453
SN - 0006-291X
VL - 167
SP - 1221
EP - 1228
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -