Extravascular optical coherence tomography: Evaluation of carotid atherosclerosis and pravastatin therapy

Robert T. Wicks, Yong Huang, Kang Zhang, Mingtao Zhao, Betty Mae Tyler, Ian Suk, Lee Hwang, Jacob Ruzevick, George Jallo, Henry Brem, Gustavo Pradilla, Jin U. Kang

Research output: Contribution to journalArticle

Abstract

BACKGROUND AND PURPOSE - : Extravascular optical coherence tomography (OCT), as a noninvasive imaging methodology with micrometer resolution, was evaluated in a murine model of carotid atherosclerosis by way of assessing the efficacy of pravastatin therapy. METHODS - : An OCT device was engineered for extravascular plaque imaging. Wild-type mice and apolipoprotein E-deficient (ApoE) mice were randomized to 3 treatment groups: (1) wild-type on a diet of standard rodent chow (n=13); (2) ApoE on a high-fat, atherosclerotic diet (HFD; n=13); and (3) ApoE on a HFD given daily pravastatin (n=13). Mice were anesthetized and the left common carotid was surgically exposed. Three-dimensional (3D; 2 spatial dimensions+time) and 4D (3 spatial dimensions+time) OCT images of the vessel lumen patency were evaluated. After perfusion, in situ OCT imaging was performed for statistical comparison with the in vivo results and final histology. RESULTS - : Intraoperative OCT imaging positively identified carotid plaque in 100% of ApoE mice on HFD. ApoE mice on HFD had a significantly decreased lumen patency when compared with that in wild-type mice (P

Original languageEnglish (US)
Pages (from-to)1123-1130
Number of pages8
JournalStroke
Volume45
Issue number4
DOIs
StatePublished - 2014

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Pravastatin
Carotid Artery Diseases
Optical Coherence Tomography
Apolipoproteins E
Therapeutics
Apolipoprotein E2
Apolipoprotein E3
High Fat Diet
Rodentia
Histology
Perfusion
Diet
Equipment and Supplies

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing

Cite this

Extravascular optical coherence tomography : Evaluation of carotid atherosclerosis and pravastatin therapy. / Wicks, Robert T.; Huang, Yong; Zhang, Kang; Zhao, Mingtao; Tyler, Betty Mae; Suk, Ian; Hwang, Lee; Ruzevick, Jacob; Jallo, George; Brem, Henry; Pradilla, Gustavo; Kang, Jin U.

In: Stroke, Vol. 45, No. 4, 2014, p. 1123-1130.

Research output: Contribution to journalArticle

Wicks, RT, Huang, Y, Zhang, K, Zhao, M, Tyler, BM, Suk, I, Hwang, L, Ruzevick, J, Jallo, G, Brem, H, Pradilla, G & Kang, JU 2014, 'Extravascular optical coherence tomography: Evaluation of carotid atherosclerosis and pravastatin therapy', Stroke, vol. 45, no. 4, pp. 1123-1130. https://doi.org/10.1161/STROKEAHA.113.002970
Wicks, Robert T. ; Huang, Yong ; Zhang, Kang ; Zhao, Mingtao ; Tyler, Betty Mae ; Suk, Ian ; Hwang, Lee ; Ruzevick, Jacob ; Jallo, George ; Brem, Henry ; Pradilla, Gustavo ; Kang, Jin U. / Extravascular optical coherence tomography : Evaluation of carotid atherosclerosis and pravastatin therapy. In: Stroke. 2014 ; Vol. 45, No. 4. pp. 1123-1130.
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AU - Zhao, Mingtao

AU - Tyler, Betty Mae

AU - Suk, Ian

AU - Hwang, Lee

AU - Ruzevick, Jacob

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AU - Brem, Henry

AU - Pradilla, Gustavo

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