Extravascular optical coherence tomography: Evaluation of carotid atherosclerosis and pravastatin therapy

Robert T. Wicks, Yong Huang, Kang Zhang, Mingtao Zhao, Betty M. Tyler, Ian Suk, Lee Hwang, Jacob Ruzevick, George Jallo, Henry Brem, Gustavo Pradilla, Jin U. Kang

Research output: Contribution to journalArticlepeer-review

Abstract

BACKGROUND AND PURPOSE - : Extravascular optical coherence tomography (OCT), as a noninvasive imaging methodology with micrometer resolution, was evaluated in a murine model of carotid atherosclerosis by way of assessing the efficacy of pravastatin therapy. METHODS - : An OCT device was engineered for extravascular plaque imaging. Wild-type mice and apolipoprotein E-deficient (ApoE) mice were randomized to 3 treatment groups: (1) wild-type on a diet of standard rodent chow (n=13); (2) ApoE on a high-fat, atherosclerotic diet (HFD; n=13); and (3) ApoE on a HFD given daily pravastatin (n=13). Mice were anesthetized and the left common carotid was surgically exposed. Three-dimensional (3D; 2 spatial dimensions+time) and 4D (3 spatial dimensions+time) OCT images of the vessel lumen patency were evaluated. After perfusion, in situ OCT imaging was performed for statistical comparison with the in vivo results and final histology. RESULTS - : Intraoperative OCT imaging positively identified carotid plaque in 100% of ApoE mice on HFD. ApoE mice on HFD had a significantly decreased lumen patency when compared with that in wild-type mice (P<0.001). Pravastatin therapy was found to increase lumen patency significantly in ApoE mice on HFD (P<0.01; compared with ApoE on HFD). The findings were confirmed with OCT imaging after perfusion and histology. CONCLUSIONS - : OCT imaging offers the potential for real-time, detailed vessel lumen evaluation, potentially improving surgical accuracy and outcomes during cerebrovascular neurosurgical procedures. Pravastatin significantly increases vessel lumen patency in the ApoE mouse on HFD.

Original languageEnglish (US)
Pages (from-to)1123-1130
Number of pages8
JournalStroke
Volume45
Issue number4
DOIs
StatePublished - Apr 2014

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

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