Extracranial measurements of amide proton transfer using exchange-modulated point-resolved spectroscopy (EXPRESS)

Simon Walker-Samuel, S. Peter Johnson, Barbara Pedley, Mark F. Lythgoe, Xavier Golay

Research output: Contribution to journalArticle

Abstract

Chemical exchange saturation transfer (CEST) imaging has been used experimentally in a large range of applications. However, full quantification of CEST effects invivo using standard imaging sequences is time consuming as a large number of saturation frequency offsets, each followed by an imaging readout, are required to define a z spectrum. Furthermore, outside the brain, the presence of fat can confound the interpretation of z spectra. A novel acquisition and post-processing technique is presented in this study, named exchange-modulated point-resolved spectroscopy (EXPRESS), which aims to address these limitations and to enable spatially localised, high signal-to-noise measurements of CEST effects invivo. Using amide proton exchange (APT) measurements in tumours, it is demonstrated that the acquisition of two-dimensional EXPRESS spectra composed of chemical shift and saturation frequency offset dimensions allows the correction of CEST data containing both fat and water signals, which is a common confounding property of tissues found outside the brain.

Original languageEnglish (US)
Pages (from-to)829-834
Number of pages6
JournalNMR in Biomedicine
Volume25
Issue number6
DOIs
StatePublished - Jun 2012
Externally publishedYes

Fingerprint

Proton transfer
Amides
Protons
Spectrum Analysis
Ion exchange
Fats
Post and Core Technique
Spectroscopy
Imaging techniques
Brain
Noise
Chemical shift
Data transfer
Water
Tumors
Tissue
Neoplasms
Processing

Keywords

  • Amide proton transfer (APT)
  • Chemical exchange saturation transfer (CEST)
  • Extracranial
  • Fat
  • MRI
  • Protein concentration
  • Tumour
  • Xenograft

ASJC Scopus subject areas

  • Spectroscopy
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Extracranial measurements of amide proton transfer using exchange-modulated point-resolved spectroscopy (EXPRESS). / Walker-Samuel, Simon; Johnson, S. Peter; Pedley, Barbara; Lythgoe, Mark F.; Golay, Xavier.

In: NMR in Biomedicine, Vol. 25, No. 6, 06.2012, p. 829-834.

Research output: Contribution to journalArticle

Walker-Samuel, Simon ; Johnson, S. Peter ; Pedley, Barbara ; Lythgoe, Mark F. ; Golay, Xavier. / Extracranial measurements of amide proton transfer using exchange-modulated point-resolved spectroscopy (EXPRESS). In: NMR in Biomedicine. 2012 ; Vol. 25, No. 6. pp. 829-834.
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