Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

S. T. Butera, V. L. Perez, N. J. Besansky, W. C. Chan, B. Y. Wu, G. J. Nabel, T. M. Folks

Research output: Contribution to journalArticlepeer-review

Abstract

In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cellls. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to + cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was

Original languageEnglish (US)
Pages (from-to)366-373
Number of pages8
JournalJournal of Cellular Biochemistry
Volume45
Issue number4
StatePublished - 1991
Externally publishedYes

Keywords

  • Azidothymidine
  • CD4
  • Clonal analysis
  • HIV-1 infection of promyelocytes
  • Unintegrated HIV-1 DNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells'. Together they form a unique fingerprint.

Cite this