Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

S. T. Butera; V. L. Perez; N. J. Besansky; W. C. Chan; B. Y. Wu; G. J. Nabel; T. M. Folks

Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

S. T. Butera, V. L. Perez, N. J. Besansky, W. C. Chan, B. Y. Wu, G. J. Nabel, T. M. Folks

Research output: Contribution to journal › Article › peer-review

Abstract

In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cellls. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to + cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1⁺ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was

Original language	English (US)
Pages (from-to)	366-373
Number of pages	8
Journal	Journal of Cellular Biochemistry
Volume	45
Issue number	4
State	Published - 1991
Externally published	Yes

Keywords

Azidothymidine
CD4
Clonal analysis
HIV-1 infection of promyelocytes
Unintegrated HIV-1 DNA

ASJC Scopus subject areas

Biochemistry
Cell Biology

Cite this

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title = "Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells",

abstract = "In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cellls. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to + cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was ",

keywords = "Azidothymidine, CD4, Clonal analysis, HIV-1 infection of promyelocytes, Unintegrated HIV-1 DNA",

author = "Butera, {S. T.} and Perez, {V. L.} and Besansky, {N. J.} and Chan, {W. C.} and Wu, {B. Y.} and Nabel, {G. J.} and Folks, {T. M.}",

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volume = "45",

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journal = "Journal of Cellular Biochemistry",

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T1 - Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

AU - Butera, S. T.

AU - Perez, V. L.

AU - Besansky, N. J.

AU - Chan, W. C.

AU - Wu, B. Y.

AU - Nabel, G. J.

AU - Folks, T. M.

PY - 1991

Y1 - 1991

N2 - In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cellls. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to + cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was

AB - In this report, we describe a human immunodeficiency virus type-1 (HIV-1)-infected promyelocytic cell line, OM, derived from HL-60 cellls. Although the OM cell line was biologically cloned twice, the pattern of HIV-1 expression during culture appeared analogous to a classical acute spreading infection and was inhibited by both azidothymidine and recombinant soluble CD4 treatment. The number of OM cells actually expressing HIV-1 at the beginning of culture was 0%, reached a peak of nearly 100% at 6 weeks, and then fell to + cells by 10 weeks. Clonal analysis of the surviving cells verified that stable HIV-1+ OM cells resulted from the spreading infection. Southern analysis confirmed the transmission of HIV-1 through these OM cultures and the occurrence of stable clones which resulted. The initial percentage of OM cells actually harboring the HIV-1 genome was

KW - Azidothymidine

KW - CD4

KW - Clonal analysis

KW - HIV-1 infection of promyelocytes

KW - Unintegrated HIV-1 DNA

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Extrachromosomal human immunodeficiency virus type-1 DNA can initiate a spreading infection of HL-60 cells

Abstract

Keywords

ASJC Scopus subject areas

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