TY - JOUR
T1 - Extracellular vesicles from different pneumococcal serotypes are internalized by macrophages and induce host immune responses
AU - Olaya-Abril, Alfonso
AU - Prados-Rosales, Rafael
AU - González-Reyes, José A.
AU - Casadevall, Arturo
AU - Pirofski, Liise Anne
AU - Rodríguez-Ortega, Manuel J.
N1 - Funding Information:
Funding: This research was funded by Programas Propios de Investigación 2017 and 2021 from University of Córdoba (MOD4.2 AGR164-AGR256 and SUBMOD2.1 respectively) to MJRO. RP-R acknowledges funding from MICINN PID2019-110240RB. L.P. acknowledges funding from National Institutes of Health grants R01AG045044 and R01AI123654.
Funding Information:
This research was funded by Programas Propios de Investigaci?n 2017 and 2021 from University of C?rdoba (MOD4.2 AGR164-AGR256 and SUBMOD2.1 respectively) to MJRO. RP-R acknowledges funding from MICINN PID2019-110240RB. L.P. acknowledges funding from National Institutes of Health grants R01AG045044 and R01AI123654.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - Bacterial extracellular vesicles are membranous ultrastructures released from the cell surface. They play important roles in the interaction between the host and the bacteria. In this work, we show how extracellular vesicles produced by four different serotypes of the important human pathogen, Streptococcus pneumoniae, are internalized by murine J774A.1 macrophages via fusion with the membrane of the host cells. We also evaluated the capacity of pneumococcal extracellular vesicles to elicit an immune response by macrophages. Macrophages treated with the vesicles underwent a serotype-dependent transient loss of viability, which was further reverted. The vesicles induced the production of proinflammatory cytokines, which was higher for serotype 1 and serotype 8-derived vesicles. These results demonstrate the biological activity of extracellular vesicles of clinically important pneumococcal serotypes.
AB - Bacterial extracellular vesicles are membranous ultrastructures released from the cell surface. They play important roles in the interaction between the host and the bacteria. In this work, we show how extracellular vesicles produced by four different serotypes of the important human pathogen, Streptococcus pneumoniae, are internalized by murine J774A.1 macrophages via fusion with the membrane of the host cells. We also evaluated the capacity of pneumococcal extracellular vesicles to elicit an immune response by macrophages. Macrophages treated with the vesicles underwent a serotype-dependent transient loss of viability, which was further reverted. The vesicles induced the production of proinflammatory cytokines, which was higher for serotype 1 and serotype 8-derived vesicles. These results demonstrate the biological activity of extracellular vesicles of clinically important pneumococcal serotypes.
KW - Host-pathogen interaction
KW - Immune response
KW - Membrane vesicles
KW - Streptococcus pneumoniae
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U2 - 10.3390/pathogens10121530
DO - 10.3390/pathogens10121530
M3 - Article
C2 - 34959485
AN - SCOPUS:85120058688
SN - 2076-0817
VL - 10
JO - Pathogens
JF - Pathogens
IS - 12
M1 - 1530
ER -