Extracellular matrix substrata alter adipocyte yield and lipogenesis in primary cultures of stromal-vascular cells from human adipose

Kim C. O'Connor, Hong Song, Nitsa Rosenzweig, David A. Jansen

Research output: Contribution to journalArticle

Abstract

The stromal-vascular fraction of human adipose was subjected to in vitro adipogenesis on different extracellular matrix substrata. Adipose tissue was harvested from the breast of 25 to 45 year-old female patients undergoing elective surgery. After 24 d, less than 5% of stromal-vascular cells had converted to adipocytes on fibronectin, 13% to 28% on tissue culture plastic and collagen I; and 59% ± 7% on Matrigel. Lipid volume surpassed 4.5 x 103 μm3 cell-1 for Matrigel and was 30% lower for the other substrata. Cell proliferation was evident for Matrigel and fibronectin, and cell spreading was most pronounced for fibronectin with a projected area exceeding 3 x 103 μm2 cell -1. These results are relevant to the design of an adipose implant, providing insight into its feasibility and scaffold composition.

Original languageEnglish (US)
Pages (from-to)1967-1972
Number of pages6
JournalBiotechnology Letters
Volume25
Issue number23
DOIs
StatePublished - Dec 1 2003

Keywords

  • Adipocytes
  • Collagen
  • Differentiation
  • Extracellular matrix
  • Fibronectin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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