Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan

Ayla O. Sessions, Gaurav Kaushik, Sarah Parker, Koen Raedschelders, Rolf Bodmer, Jennifer E. Van Eyk, Adam J. Engler

Research output: Contribution to journalArticle

Abstract

Aging is associated with extensive remodeling of the heart, including basement membrane (BM) components that surround cardiomyocytes. Remodeling is thought to impair cardiac mechanotransduction, but the contribution of specific BM components to age-related lateral communication between cardiomyocytes is unclear. Using a genetically tractable, rapidly aging model with sufficient cardiac genetic homology and morphology, e.g. . Drosophila melanogaster, we observed differential regulation of BM collagens between laboratory strains, correlating with changes in muscle physiology leading to cardiac dysfunction. Therefore, we sought to understand the extent to which BM proteins modulate contractile function during aging. Cardiac-restricted knockdown of ECM genes . Pericardin, . Laminin A, and . Viking in . Drosophila prevented age-associated heart tube restriction and increased contractility, even under viscous load. Most notably, reduction of . Laminin A expression correlated with an overall preservation of contractile velocity with age and extension of organismal lifespan. Global heterozygous knockdown confirmed these data, which provides new evidence of a direct link between BM homeostasis, contractility, and maintenance of lifespan.

Original languageEnglish (US)
JournalMatrix Biology
DOIs
StateAccepted/In press - Sep 23 2016
Externally publishedYes

Fingerprint

Basement Membrane
Drosophila
Extracellular Matrix
Down-Regulation
Cardiac Myocytes
Gene Knockdown Techniques
Drosophila melanogaster
Membrane Proteins
Homeostasis
Collagen
Communication
Maintenance
Muscles
laminin A

Keywords

  • Aging
  • Basement membrane
  • Drosophila
  • Extracellular matrix
  • Laminin A

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Sessions, A. O., Kaushik, G., Parker, S., Raedschelders, K., Bodmer, R., Van Eyk, J. E., & Engler, A. J. (Accepted/In press). Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan. Matrix Biology. https://doi.org/10.1016/j.matbio.2016.10.008

Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan. / Sessions, Ayla O.; Kaushik, Gaurav; Parker, Sarah; Raedschelders, Koen; Bodmer, Rolf; Van Eyk, Jennifer E.; Engler, Adam J.

In: Matrix Biology, 23.09.2016.

Research output: Contribution to journalArticle

Sessions, Ayla O. ; Kaushik, Gaurav ; Parker, Sarah ; Raedschelders, Koen ; Bodmer, Rolf ; Van Eyk, Jennifer E. ; Engler, Adam J. / Extracellular matrix downregulation in the Drosophila heart preserves contractile function and improves lifespan. In: Matrix Biology. 2016.
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