Extracellular human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-κB binding and protein kinase C activity in primary human astrocytes

Katherine Conant, Methui Ma, Avindra Nath, Eugene O. Major

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus type 1 (HIV-1) infection has been associated with an increase in the binding of the transcription factor NF-κB to its consensus sequence in the viral promoter. Using cultures of primary human fetal astrocytes, we show that exogenous HIV-1 Tat protein, which has been demonstrated to be released from infected cells, is associated with an increase in the binding of this transcription factor to an HIV-1 long terminal repeat κB sequence. This effect occurs rapidly and is independent of new protein synthesis. We also demonstrate that extracellular Tat protein is associated with an increase in protein kinase C activity. If Tat functions similarly in other cell types, such findings could relate to some of this protein's previously described physiological effects. These effects include Tat's ability to upregulate the synthesis of specific cytokines and to act as a growth factor.

Original languageEnglish (US)
Pages (from-to)1384-1389
Number of pages6
JournalJournal of Virology
Volume70
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

tat Gene Products
astrocytes
protein kinase C
Human immunodeficiency virus 1
Astrocytes
Protein Kinase C
HIV-1
Carrier Proteins
Transcription Factors
transcription factors
HIV Long Terminal Repeat
terminal repeat sequences
proteins
Terminal Repeat Sequences
consensus sequence
Consensus Sequence
Virus Diseases
growth factors
Intercellular Signaling Peptides and Proteins
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Extracellular human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-κB binding and protein kinase C activity in primary human astrocytes. / Conant, Katherine; Ma, Methui; Nath, Avindra; Major, Eugene O.

In: Journal of Virology, Vol. 70, No. 3, 1996, p. 1384-1389.

Research output: Contribution to journalArticle

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