Extracellular cAMP is sufficient to restore developmental gene expression and morphogenesis in Dictyostelium cells lacking the aggregation adenylyl cyclase (ACA)

Geoffrey S. Pitt, Raymond Brandt, Kenneth C. Lin, Peter N. Devreotes, Pauline Schaap

Research output: Contribution to journalArticlepeer-review

Abstract

Cell movement and cell-type-specific gene expression during Dictyostelium development are regulated by cAMF, which functions both as an extracellular hormone-like signal and an intracellular second messenger. Previous data indicated that aca- mutants, which lack adenylyl cyclase activity, fail to aggregate and do not express cell-type-specific genes. We show here that overexpression of ACG, a constitutively active adenylyl cyclase, which in wild-type cells is only expressed during spore germination, partially restores the coordination of cell movement and completely restores developmental gene expression. The aca- cells can also be induced to develop into viable spores by synergy with wild-type cells and, furthermore, form small but normal fruiting bodies, after a developmentally relevant regimen of stimulation with nanomolar cAMF pulses followed by micromolar cAMP concentrations. 2′-Deoxy cAMP, a cAMP analog that activates the cell-surface cAMP receptors but not cAMP-dependent protein kinase (PKA), also induces fruiting body formation as well as expression of prespore-specific and prestalk-enriched genes in aca- cells. Intracellular cAMP levels were not altered in aca- cells after stimulation with 2′-deoxy cAMP. Our data indicate that ACA is not required to provide intracellular cAMP for PKA activation but is essential to produce extracellular cAMP for coordination of cell movement during all stages of development and for induction of developmental gene expression.

Original languageEnglish (US)
Pages (from-to)2172-2180
Number of pages9
JournalGenes and Development
Volume7
Issue number11
DOIs
StatePublished - 1993

Keywords

  • Adenylyl cyclase
  • Developmental gene expression
  • Dictyostelium discoideum
  • Gene disruption
  • Morphogenesis
  • cAMP receptor

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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