Extension of the 2-deoxyglucose method to the fetus in utero: Theory and normal values for the cerebral glucose consumption in fetal guinea pigs

Richard Berger, Albert Gjedde, Johannes Heck, Elke Müller, Josef Krieglstein, Arne Jensen

Research output: Contribution to journalArticle

Abstract

Fetal cerebral metabolism changes during development. The normal fetal metabolic rate must be known to evaluate pathophysiological changes. Therefore, we determined the regional cerebral glucose consumption in the fetal guinea pig. This required the application of the 2-deoxyglucose method to this species. We measured both the transfer coefficients of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the lumped constant in chronically prepared undisturbed guinea pig dams using a three-compartment model. Furthermore, the ratio between the initial clearances of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the ratio between the phosphorylation coefficients of these substrates in the fetal brain were determined. The total cerebral glucose consumption measured by the deoxyglucose method (10 ± 1.2 μmol/100 g/min) was similar to that calculated from the glucose concentration and the phosphorylation coefficient of glucose in the cerebrum (10 ± 0.4 μmol/100 g/min). We conclude that the 2-deoxyglucose method is applicable to the guinea pig, and we further conclude that in the fetal guinea pig cerebral glucose consumption is 10 times lower than that in the glucose consumption is 10 times lower than that in the adult.

Original languageEnglish (US)
Pages (from-to)271-279
Number of pages9
JournalJournal of Neurochemistry
Volume63
Issue number1
StatePublished - Jul 1994
Externally publishedYes

Fingerprint

Deoxyglucose
Guinea Pigs
Reference Values
Fetus
Glucose
Brain
Phosphorylation
Mothers
Plasmas
Cerebrum
Metabolism
Dams
Substrates

Keywords

  • Cerebral metabolism
  • Deoxyglucose
  • Fetal guinea pig
  • Lumped constant

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Extension of the 2-deoxyglucose method to the fetus in utero : Theory and normal values for the cerebral glucose consumption in fetal guinea pigs. / Berger, Richard; Gjedde, Albert; Heck, Johannes; Müller, Elke; Krieglstein, Josef; Jensen, Arne.

In: Journal of Neurochemistry, Vol. 63, No. 1, 07.1994, p. 271-279.

Research output: Contribution to journalArticle

Berger, Richard ; Gjedde, Albert ; Heck, Johannes ; Müller, Elke ; Krieglstein, Josef ; Jensen, Arne. / Extension of the 2-deoxyglucose method to the fetus in utero : Theory and normal values for the cerebral glucose consumption in fetal guinea pigs. In: Journal of Neurochemistry. 1994 ; Vol. 63, No. 1. pp. 271-279.
@article{ec35aae50d6e48f09310bbe30b8062b7,
title = "Extension of the 2-deoxyglucose method to the fetus in utero: Theory and normal values for the cerebral glucose consumption in fetal guinea pigs",
abstract = "Fetal cerebral metabolism changes during development. The normal fetal metabolic rate must be known to evaluate pathophysiological changes. Therefore, we determined the regional cerebral glucose consumption in the fetal guinea pig. This required the application of the 2-deoxyglucose method to this species. We measured both the transfer coefficients of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the lumped constant in chronically prepared undisturbed guinea pig dams using a three-compartment model. Furthermore, the ratio between the initial clearances of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the ratio between the phosphorylation coefficients of these substrates in the fetal brain were determined. The total cerebral glucose consumption measured by the deoxyglucose method (10 ± 1.2 μmol/100 g/min) was similar to that calculated from the glucose concentration and the phosphorylation coefficient of glucose in the cerebrum (10 ± 0.4 μmol/100 g/min). We conclude that the 2-deoxyglucose method is applicable to the guinea pig, and we further conclude that in the fetal guinea pig cerebral glucose consumption is 10 times lower than that in the glucose consumption is 10 times lower than that in the adult.",
keywords = "Cerebral metabolism, Deoxyglucose, Fetal guinea pig, Lumped constant",
author = "Richard Berger and Albert Gjedde and Johannes Heck and Elke M{\"u}ller and Josef Krieglstein and Arne Jensen",
year = "1994",
month = "7",
language = "English (US)",
volume = "63",
pages = "271--279",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Extension of the 2-deoxyglucose method to the fetus in utero

T2 - Theory and normal values for the cerebral glucose consumption in fetal guinea pigs

AU - Berger, Richard

AU - Gjedde, Albert

AU - Heck, Johannes

AU - Müller, Elke

AU - Krieglstein, Josef

AU - Jensen, Arne

PY - 1994/7

Y1 - 1994/7

N2 - Fetal cerebral metabolism changes during development. The normal fetal metabolic rate must be known to evaluate pathophysiological changes. Therefore, we determined the regional cerebral glucose consumption in the fetal guinea pig. This required the application of the 2-deoxyglucose method to this species. We measured both the transfer coefficients of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the lumped constant in chronically prepared undisturbed guinea pig dams using a three-compartment model. Furthermore, the ratio between the initial clearances of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the ratio between the phosphorylation coefficients of these substrates in the fetal brain were determined. The total cerebral glucose consumption measured by the deoxyglucose method (10 ± 1.2 μmol/100 g/min) was similar to that calculated from the glucose concentration and the phosphorylation coefficient of glucose in the cerebrum (10 ± 0.4 μmol/100 g/min). We conclude that the 2-deoxyglucose method is applicable to the guinea pig, and we further conclude that in the fetal guinea pig cerebral glucose consumption is 10 times lower than that in the glucose consumption is 10 times lower than that in the adult.

AB - Fetal cerebral metabolism changes during development. The normal fetal metabolic rate must be known to evaluate pathophysiological changes. Therefore, we determined the regional cerebral glucose consumption in the fetal guinea pig. This required the application of the 2-deoxyglucose method to this species. We measured both the transfer coefficients of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the lumped constant in chronically prepared undisturbed guinea pig dams using a three-compartment model. Furthermore, the ratio between the initial clearances of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the ratio between the phosphorylation coefficients of these substrates in the fetal brain were determined. The total cerebral glucose consumption measured by the deoxyglucose method (10 ± 1.2 μmol/100 g/min) was similar to that calculated from the glucose concentration and the phosphorylation coefficient of glucose in the cerebrum (10 ± 0.4 μmol/100 g/min). We conclude that the 2-deoxyglucose method is applicable to the guinea pig, and we further conclude that in the fetal guinea pig cerebral glucose consumption is 10 times lower than that in the glucose consumption is 10 times lower than that in the adult.

KW - Cerebral metabolism

KW - Deoxyglucose

KW - Fetal guinea pig

KW - Lumped constant

UR - http://www.scopus.com/inward/record.url?scp=0028234035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028234035&partnerID=8YFLogxK

M3 - Article

C2 - 8207433

AN - SCOPUS:0028234035

VL - 63

SP - 271

EP - 279

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 1

ER -