Extending the cytoplasmic tail of the influenza A virus M2 protein leads to reduced virus replication in vivo but not in vitro

Wai Hong Wu, Andrew Pekosz

Research output: Contribution to journalArticle

Abstract

A carboxy-terminal epitope tag introduced into the coding region of the A/WSN/33 M2 protein resulted in a recombinant virus (rWSN M2myc) which replicated to titers similar to those of the parental virus (rWSN) in MDCK cells. The rWSN M2myc virus was attenuated in its ability to induce mortality and weight loss after the intranasal inoculation of BALB/c mice, indicating that the M2 cytoplasmic tail plays a role in virus virulence. Mice infected with rWSN M2myc were completely protected from subsequent challenge with rWSN, suggesting that epitope tagging of the M2 protein may be a useful way of attenuating influenza A virus strains.

Original languageEnglish (US)
Pages (from-to)1059-1063
Number of pages5
JournalJournal of virology
Volume82
Issue number2
DOIs
StatePublished - Jan 1 2008

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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