Expressional difference, distributions of TGF-β1 in TGF-β1 knock down transgenic mouse, and its possible roles in injured spinal cord

Yan Bin XiYang, Bing Tuan Lu, [No Value] Ya-Zhao, [No Value] Yuan-Zhang, Qin Jie Xia, Yu Zou, Wei Zhang, Xiong Zhi Quan, Su Liu, John W. McDonald, Lian Feng Zhang, Ting Hua Wang

Research output: Contribution to journalArticle

Abstract

Transforming growth factor β1 (TGF-β1) is a multi-functional cytokine implicated in many aspects of mammalian wound healing and scar tissue formation. However, few experiments have so far addressed the potential biological effects of TGF-β1 in the nervous system after injury, in addition to the immune system. In the present study, expressional silencing TGF-β1 was achieved by selecting predesigning hairpins targeting mouse TGF-β1 genes. Four homozygous transgenic offspring were generated and designed as Founder 90, Founder 12, Founder 41 and Founder 46. The down-regulated rates of TGF-β1 in different transgenic mice were also determined. To investigate the potential roles of TGF-β1, we observed changes in the neurological behavior of TGF-β1-knockdown (TGF-β1-kd) mice after spinal cord transection (SCT). Moreover, mRNA levels of inflammatory cytokines, including IL-1, IL-6, IL-10, NF-κB and TNF, were also detected in nucleate cells from blood by real-time PCR. Consequently, different TGF-β1 expressions were detected in multiple tissues, and protein levels of TGF-β1 decreased at different rates relative to that of wild type (WT) ones. The levels of TGF-β1 proteins in TGF-β1-kd mice decreased at most by 57% in Founder 90, which showed a significant recovery in Basso, Beattie, Bresnahan (BBB) scores after SCT compared with that of WT. However, expressions of immune relative genes showed no dramatic difference compared with WT ones. This study is the first to generate TGF-β1 down regulated mice and determine the possible roles of TGF-β1 in vivo in different conditions.

Original languageEnglish (US)
Pages (from-to)320-329
Number of pages10
JournalExperimental Biology and Medicine
Volume239
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Transforming Growth Factors
Transgenic Mice
Spinal Cord
Spinal Cord Injuries
Genes
Tissue
Cytokines
Nervous System Trauma
Immune system
Neurology
Interleukin-1
Interleukin-10
Wound Healing
Cicatrix
Real-Time Polymerase Chain Reaction
Immune System
Interleukin-6
Blood Cells
Proteins
Blood

Keywords

  • distribution and localization
  • knockdown
  • phenotype
  • protein expression
  • TGF-β1
  • transgenic mouse

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Expressional difference, distributions of TGF-β1 in TGF-β1 knock down transgenic mouse, and its possible roles in injured spinal cord. / XiYang, Yan Bin; Lu, Bing Tuan; Ya-Zhao, [No Value]; Yuan-Zhang, [No Value]; Xia, Qin Jie; Zou, Yu; Zhang, Wei; Quan, Xiong Zhi; Liu, Su; McDonald, John W.; Zhang, Lian Feng; Wang, Ting Hua.

In: Experimental Biology and Medicine, Vol. 239, No. 3, 2014, p. 320-329.

Research output: Contribution to journalArticle

XiYang, YB, Lu, BT, Ya-Zhao, NV, Yuan-Zhang, NV, Xia, QJ, Zou, Y, Zhang, W, Quan, XZ, Liu, S, McDonald, JW, Zhang, LF & Wang, TH 2014, 'Expressional difference, distributions of TGF-β1 in TGF-β1 knock down transgenic mouse, and its possible roles in injured spinal cord', Experimental Biology and Medicine, vol. 239, no. 3, pp. 320-329. https://doi.org/10.1177/1535370213509562
XiYang, Yan Bin ; Lu, Bing Tuan ; Ya-Zhao, [No Value] ; Yuan-Zhang, [No Value] ; Xia, Qin Jie ; Zou, Yu ; Zhang, Wei ; Quan, Xiong Zhi ; Liu, Su ; McDonald, John W. ; Zhang, Lian Feng ; Wang, Ting Hua. / Expressional difference, distributions of TGF-β1 in TGF-β1 knock down transgenic mouse, and its possible roles in injured spinal cord. In: Experimental Biology and Medicine. 2014 ; Vol. 239, No. 3. pp. 320-329.
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