Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice

Ya Yun Wang; Yan Yan Wei; Jing Huang; Wei Wang; Nobuaiki Tamamaki; Yun Qing Li; Sheng Xi Wu

doi:10.1016/j.jchemneu.2009.04.003

Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD₆₇-GFP knock-in mice

Ya Yun Wang, Yan Yan Wei, Jing Huang, Wei Wang, Nobuaiki Tamamaki, Yun Qing Li, Sheng Xi Wu

Research output: Contribution to journal › Article › peer-review

Abstract

5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD₆₇-GFP knock-in mice. The present result showed that 5-HT_1A receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT_1A receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT_2A receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT_2A receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.

Original language	English (US)
Pages (from-to)	75-81
Number of pages	7
Journal	Journal of Chemical Neuroanatomy
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.jchemneu.2009.04.003
State	Published - Sep 2009
Externally published	Yes

Keywords

Descending antinociceptive system
Double immunofluorescent staining
GABAergic interneuron
Nociceptive modulation
Single-cell RT-PCR

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

Access to Document

10.1016/j.jchemneu.2009.04.003

Cite this

Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD₆₇-GFP knock-in mice. / Wang, Ya Yun; Wei, Yan Yan; Huang, Jing; Wang, Wei; Tamamaki, Nobuaiki; Li, Yun Qing; Wu, Sheng Xi.

In: Journal of Chemical Neuroanatomy, Vol. 38, No. 1, 09.2009, p. 75-81.

Research output: Contribution to journal › Article › peer-review

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title = "Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice",

abstract = "5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD67-GFP knock-in mice. The present result showed that 5-HT1A receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT1A receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT2A receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT2A receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.",

keywords = "Descending antinociceptive system, Double immunofluorescent staining, GABAergic interneuron, Nociceptive modulation, Single-cell RT-PCR",

author = "Wang, {Ya Yun} and Wei, {Yan Yan} and Jing Huang and Wei Wang and Nobuaiki Tamamaki and Li, {Yun Qing} and Wu, {Sheng Xi}",

note = "Funding Information: We thank Prof. RW Guillery (Department of Anatomy, Marmara University, Istanbul, Turkey) for his critical reading and constructive suggestions on the manuscript. This work was supported by grants from the National Natural Science Foundation of China (Nos. 30600176, 30700811 and 30770694), The Program for New Century Excellent Talents and Innovation Research Team Program of Ministry of Education of China (IRT0560). Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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AU - Tamamaki, Nobuaiki

AU - Li, Yun Qing

AU - Wu, Sheng Xi

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