Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice

Ya Yun Wang, Yan Yan Wei, Jing Huang, Wei Wang, Nobuaiki Tamamaki, Yun Qing Li, Sheng Xi Wu

Research output: Contribution to journal › Article

Abstract

5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD67-GFP knock-in mice. The present result showed that 5-HT1A receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT1A receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT2A receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT2A receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalJournal of Chemical Neuroanatomy
Volume38
Issue number1
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

Fingerprint

Receptor, Serotonin, 5-HT2A
GABAergic Neurons
Receptor, Serotonin, 5-HT1A
Interneurons
gamma-Aminobutyric Acid
Spinal Cord
GABA Agents
Polymerase Chain Reaction
Messenger RNA
Nociception
Serotonin Receptors
Serotonin
Staining and Labeling
Population
Spinal Cord Dorsal Horn
Proteins

Keywords

  • Descending antinociceptive system
  • Double immunofluorescent staining
  • GABAergic interneuron
  • Nociceptive modulation
  • Single-cell RT-PCR

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice. / Wang, Ya Yun; Wei, Yan Yan; Huang, Jing; Wang, Wei; Tamamaki, Nobuaiki; Li, Yun Qing; Wu, Sheng Xi.

In: Journal of Chemical Neuroanatomy, Vol. 38, No. 1, 01.09.2009, p. 75-81.

Research output: Contribution to journal › Article

Wang, YY, Wei, YY, Huang, J, Wang, W, Tamamaki, N, Li, YQ & Wu, SX 2009, 'Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice', Journal of Chemical Neuroanatomy, vol. 38, no. 1, pp. 75-81. https://doi.org/10.1016/j.jchemneu.2009.04.003
Wang YY, Wei YY, Huang J, Wang W, Tamamaki N, Li YQ et al. Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice. Journal of Chemical Neuroanatomy. 2009 Sep 1;38(1):75-81. https://doi.org/10.1016/j.jchemneu.2009.04.003
Wang, Ya Yun ; Wei, Yan Yan ; Huang, Jing ; Wang, Wei ; Tamamaki, Nobuaiki ; Li, Yun Qing ; Wu, Sheng Xi. / Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice. In: Journal of Chemical Neuroanatomy. 2009 ; Vol. 38, No. 1. pp. 75-81.
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