TY - JOUR
T1 - Expression patterns of 5-HT receptor subtypes 1A and 2A on GABAergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice
AU - Wang, Ya Yun
AU - Wei, Yan Yan
AU - Huang, Jing
AU - Wang, Wei
AU - Tamamaki, Nobuaiki
AU - Li, Yun Qing
AU - Wu, Sheng Xi
N1 - Funding Information:
We thank Prof. RW Guillery (Department of Anatomy, Marmara University, Istanbul, Turkey) for his critical reading and constructive suggestions on the manuscript. This work was supported by grants from the National Natural Science Foundation of China (Nos. 30600176, 30700811 and 30770694), The Program for New Century Excellent Talents and Innovation Research Team Program of Ministry of Education of China (IRT0560).
PY - 2009/9
Y1 - 2009/9
N2 - 5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD67-GFP knock-in mice. The present result showed that 5-HT1A receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT1A receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT2A receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT2A receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.
AB - 5-HT plays an important role in GABA transmission at the spinal cord level. The main purpose of the present article is to find out, which 5-HT receptor subtypes may be involved in the regulation of GABAergic transmission in the spinal dorsal horn, by using the combination of tissue RT-PCR, single-cell RT-PCR and double immunofluorescent staining on the GAD67-GFP knock-in mice. The present result showed that 5-HT1A receptor subtype mRNA was present on about half of GFP-positive GABAergic interneurons in the spinal dorsal horn, which suggests a role of 5-HT1A receptor in GABA-mediated modulation of nociception at the spinal cord level. However, it is unexpected that just a very limited sub-population of the total GABAergic interneurons express 5-HT2A receptor mRNA or proteins in the spinal dorsal horn, which suggests that the 5-HT2A receptor subtype may not be important for spinal GABAergic effects in nociceptive modulation.
KW - Descending antinociceptive system
KW - Double immunofluorescent staining
KW - GABAergic interneuron
KW - Nociceptive modulation
KW - Single-cell RT-PCR
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U2 - 10.1016/j.jchemneu.2009.04.003
DO - 10.1016/j.jchemneu.2009.04.003
M3 - Article
C2 - 19409978
AN - SCOPUS:67549109088
VL - 38
SP - 75
EP - 81
JO - Journal of Chemical Neuroanatomy
JF - Journal of Chemical Neuroanatomy
SN - 0891-0618
IS - 1
ER -