Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene

Bhaja K. Padhi, J. Samuel Zigler, Piyush Padhi, Stacey Hose, Debasish Sinha

Research output: Contribution to journalArticle

Abstract

Summary: The transforming acidic coiled-coil containing protein 2 (Tacc2) gene and its paralogs, Tacc1 and Tacc3 encode proteins that are associated with the centrosome and involved in microtubule assembly during the cell cycle. Tacc2 produces several splice variants, which are poorly characterized, especially in the rat. Characterization of the temporal/spatial expression patterns of these isoforms would be useful in understanding their distinct and overlapping functions. By comparative sequence analyses of Tacc2 in multiple species, we identified a third splice variant in rat, which is much shorter in size (1,021 aa) than the longest isoform (2,834 aa). This newly identified Tacc2 splice variant (isoform 3) uses a distinct first exon and generates a different open reading frame. Although Isoform 3 is expressed predominantly during developmental stages, the long Tacc2 isoform (isoform 1) is distributed mainly in adult tissues. Multiple protein sequence analyses revealed that Tacc2 Isoform 3 could be the ancient form, as it is conserved in mammals, birds, and amphibians; whereas the long Tacc2 isoforms may have evolved in the mammalian lineage by adding exons toward the 5′ region of the ancient isoform. genesis 52:378-386, 2014.

Original languageEnglish (US)
Pages (from-to)378-386
Number of pages9
JournalGenesis
Volume52
Issue number5
DOIs
StatePublished - 2014

Fingerprint

Protein Isoforms
Proteins
Exons
Centrosome
Protein Sequence Analysis
Amphibians
Microtubules
Open Reading Frames
Birds
Sequence Analysis
Mammals
Cell Cycle

Keywords

  • Alterative promoter
  • Alternative splicing
  • Comparative sequence analysis

ASJC Scopus subject areas

  • Endocrinology
  • Cell Biology
  • Genetics
  • Medicine(all)

Cite this

Padhi, B. K., Zigler, J. S., Padhi, P., Hose, S., & Sinha, D. (2014). Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene. Genesis, 52(5), 378-386. https://doi.org/10.1002/dvg.22776

Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene. / Padhi, Bhaja K.; Zigler, J. Samuel; Padhi, Piyush; Hose, Stacey; Sinha, Debasish.

In: Genesis, Vol. 52, No. 5, 2014, p. 378-386.

Research output: Contribution to journalArticle

Padhi, BK, Zigler, JS, Padhi, P, Hose, S & Sinha, D 2014, 'Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene', Genesis, vol. 52, no. 5, pp. 378-386. https://doi.org/10.1002/dvg.22776
Padhi, Bhaja K. ; Zigler, J. Samuel ; Padhi, Piyush ; Hose, Stacey ; Sinha, Debasish. / Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene. In: Genesis. 2014 ; Vol. 52, No. 5. pp. 378-386.
@article{2a58d428d6e84f16a36b97dabb68d7b6,
title = "Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene",
abstract = "Summary: The transforming acidic coiled-coil containing protein 2 (Tacc2) gene and its paralogs, Tacc1 and Tacc3 encode proteins that are associated with the centrosome and involved in microtubule assembly during the cell cycle. Tacc2 produces several splice variants, which are poorly characterized, especially in the rat. Characterization of the temporal/spatial expression patterns of these isoforms would be useful in understanding their distinct and overlapping functions. By comparative sequence analyses of Tacc2 in multiple species, we identified a third splice variant in rat, which is much shorter in size (1,021 aa) than the longest isoform (2,834 aa). This newly identified Tacc2 splice variant (isoform 3) uses a distinct first exon and generates a different open reading frame. Although Isoform 3 is expressed predominantly during developmental stages, the long Tacc2 isoform (isoform 1) is distributed mainly in adult tissues. Multiple protein sequence analyses revealed that Tacc2 Isoform 3 could be the ancient form, as it is conserved in mammals, birds, and amphibians; whereas the long Tacc2 isoforms may have evolved in the mammalian lineage by adding exons toward the 5′ region of the ancient isoform. genesis 52:378-386, 2014.",
keywords = "Alterative promoter, Alternative splicing, Comparative sequence analysis",
author = "Padhi, {Bhaja K.} and Zigler, {J. Samuel} and Piyush Padhi and Stacey Hose and Debasish Sinha",
year = "2014",
doi = "10.1002/dvg.22776",
language = "English (US)",
volume = "52",
pages = "378--386",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Liss Inc.",
number = "5",

}

TY - JOUR

T1 - Expression pattern of an evolutionarily conserved splice variant in the rat Tacc2 gene

AU - Padhi, Bhaja K.

AU - Zigler, J. Samuel

AU - Padhi, Piyush

AU - Hose, Stacey

AU - Sinha, Debasish

PY - 2014

Y1 - 2014

N2 - Summary: The transforming acidic coiled-coil containing protein 2 (Tacc2) gene and its paralogs, Tacc1 and Tacc3 encode proteins that are associated with the centrosome and involved in microtubule assembly during the cell cycle. Tacc2 produces several splice variants, which are poorly characterized, especially in the rat. Characterization of the temporal/spatial expression patterns of these isoforms would be useful in understanding their distinct and overlapping functions. By comparative sequence analyses of Tacc2 in multiple species, we identified a third splice variant in rat, which is much shorter in size (1,021 aa) than the longest isoform (2,834 aa). This newly identified Tacc2 splice variant (isoform 3) uses a distinct first exon and generates a different open reading frame. Although Isoform 3 is expressed predominantly during developmental stages, the long Tacc2 isoform (isoform 1) is distributed mainly in adult tissues. Multiple protein sequence analyses revealed that Tacc2 Isoform 3 could be the ancient form, as it is conserved in mammals, birds, and amphibians; whereas the long Tacc2 isoforms may have evolved in the mammalian lineage by adding exons toward the 5′ region of the ancient isoform. genesis 52:378-386, 2014.

AB - Summary: The transforming acidic coiled-coil containing protein 2 (Tacc2) gene and its paralogs, Tacc1 and Tacc3 encode proteins that are associated with the centrosome and involved in microtubule assembly during the cell cycle. Tacc2 produces several splice variants, which are poorly characterized, especially in the rat. Characterization of the temporal/spatial expression patterns of these isoforms would be useful in understanding their distinct and overlapping functions. By comparative sequence analyses of Tacc2 in multiple species, we identified a third splice variant in rat, which is much shorter in size (1,021 aa) than the longest isoform (2,834 aa). This newly identified Tacc2 splice variant (isoform 3) uses a distinct first exon and generates a different open reading frame. Although Isoform 3 is expressed predominantly during developmental stages, the long Tacc2 isoform (isoform 1) is distributed mainly in adult tissues. Multiple protein sequence analyses revealed that Tacc2 Isoform 3 could be the ancient form, as it is conserved in mammals, birds, and amphibians; whereas the long Tacc2 isoforms may have evolved in the mammalian lineage by adding exons toward the 5′ region of the ancient isoform. genesis 52:378-386, 2014.

KW - Alterative promoter

KW - Alternative splicing

KW - Comparative sequence analysis

UR - http://www.scopus.com/inward/record.url?scp=84900861018&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900861018&partnerID=8YFLogxK

U2 - 10.1002/dvg.22776

DO - 10.1002/dvg.22776

M3 - Article

C2 - 24700488

AN - SCOPUS:84900861018

VL - 52

SP - 378

EP - 386

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 5

ER -