TY - JOUR
T1 - Expression of transient receptor potential canonical channel proteins in human non-small cell lung cancer
AU - Zhang, Qi
AU - He, Jianxing
AU - Lu, Wenju
AU - Yin, Weiqiang
AU - Yang, Haihong
AU - Xu, Xiaoming
AU - Wang, Daoyuan
PY - 2010/6
Y1 - 2010/6
N2 - Background and objective: Transient receptor potential canonical (TRPC) proteins, a group of Ca2+ permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC). Methods: Real-time quantitative PCR was performed to screen the expression of TRPC mRNA in NSCLC tissue. Protein expression of TRPC was detected by Western blot. Results: Among the seven family members of TRPC so far identified (TRPC1-7), we detected the expression of TRPC1, TRPC3, TRPC4, TRPC6 mRNA in 24 cases of NSCLC tissue; TRPC2, TRPC5 and TRPC7 mRNA were not detectable. The relative abundance of the expressed TRPC was TRPC1≈TRPC6>TRPC3>TRPC4. Western blot confirmed the protein expression of TRPC1, TRPC3, TRPC4 and TRPC6 in NSCLC tissue. Conclusion: Out of the seven members of TRPC, we found TRPC1, TRPC3, TRPC4, TRPC6 mRNA and protein were selectively expressed in human NSCLC tissue. "is study could provide a basis for future exploration of the individual role of these TRPC proteins in mediating SOCE and in the progression of lung cancer.
AB - Background and objective: Transient receptor potential canonical (TRPC) proteins, a group of Ca2+ permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC). Methods: Real-time quantitative PCR was performed to screen the expression of TRPC mRNA in NSCLC tissue. Protein expression of TRPC was detected by Western blot. Results: Among the seven family members of TRPC so far identified (TRPC1-7), we detected the expression of TRPC1, TRPC3, TRPC4, TRPC6 mRNA in 24 cases of NSCLC tissue; TRPC2, TRPC5 and TRPC7 mRNA were not detectable. The relative abundance of the expressed TRPC was TRPC1≈TRPC6>TRPC3>TRPC4. Western blot confirmed the protein expression of TRPC1, TRPC3, TRPC4 and TRPC6 in NSCLC tissue. Conclusion: Out of the seven members of TRPC, we found TRPC1, TRPC3, TRPC4, TRPC6 mRNA and protein were selectively expressed in human NSCLC tissue. "is study could provide a basis for future exploration of the individual role of these TRPC proteins in mediating SOCE and in the progression of lung cancer.
KW - Lung neoplasms
KW - Store-operated calcium entry
KW - Transient receptor potential canonical proteins
UR - http://www.scopus.com/inward/record.url?scp=77953622999&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953622999&partnerID=8YFLogxK
U2 - 10.3779/j.issn.1009-3419.2010.06.009
DO - 10.3779/j.issn.1009-3419.2010.06.009
M3 - Article
C2 - 20681449
AN - SCOPUS:77953622999
VL - 13
SP - 612
EP - 616
JO - Chinese Journal of Lung Cancer
JF - Chinese Journal of Lung Cancer
SN - 1009-3419
IS - 6
ER -