Expression of transient receptor potential canonical channel proteins in human non-small cell lung cancer

Qi Zhang, Jianxing He, Wenju Lu, Weiqiang Yin, Haihong Yang, Xiaoming Xu, Daoyuan Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Background and objective: Transient receptor potential canonical (TRPC) proteins, a group of Ca2+ permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC). Methods: Real-time quantitative PCR was performed to screen the expression of TRPC mRNA in NSCLC tissue. Protein expression of TRPC was detected by Western blot. Results: Among the seven family members of TRPC so far identified (TRPC1-7), we detected the expression of TRPC1, TRPC3, TRPC4, TRPC6 mRNA in 24 cases of NSCLC tissue; TRPC2, TRPC5 and TRPC7 mRNA were not detectable. The relative abundance of the expressed TRPC was TRPC1≈TRPC6>TRPC3>TRPC4. Western blot confirmed the protein expression of TRPC1, TRPC3, TRPC4 and TRPC6 in NSCLC tissue. Conclusion: Out of the seven members of TRPC, we found TRPC1, TRPC3, TRPC4, TRPC6 mRNA and protein were selectively expressed in human NSCLC tissue. "is study could provide a basis for future exploration of the individual role of these TRPC proteins in mediating SOCE and in the progression of lung cancer.

Original languageEnglish (US)
Pages (from-to)612-616
Number of pages5
JournalChinese Journal of Lung Cancer
Volume13
Issue number6
DOIs
StatePublished - Jun 2010

Keywords

  • Lung neoplasms
  • Store-operated calcium entry
  • Transient receptor potential canonical proteins

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

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