Expression of the kynurenine pathway enzyme tryptophan 2,3-dioxygenase is increased in the frontal cortex of individuals with schizophrenia

Christine L. Miller, Ida C. Llenos, Jeanette R. Dulay, Meliza M. Barillo, Robert H. Yolken, Serge Weis

Research output: Contribution to journalArticle

Abstract

Markers of the kynurenine pathway were studied in postmortem frontal cortex obtained from individuals with schizophrenia and controls. Quantitative endpoint RT-PCR was used to measure mRNA transcripts. Of the two enzymes capable of catalyzing the first step in the pathway, tryptophan 2,3-dioxygenase (TDO2) and indoleamine dioxygenase (IDO), the concentration of mRNA for TDO2 was found to be elevated 1.6-fold in the schizophrenia group (P = 0.03), whereas the concentration of the mRNA for IDO was not significantly different between the schizophrenia and control groups. Immunohistochemistry showed an increased density of TDO2-immunopositive astroglial cells in the white matter of patients with schizophrenia (P = 0.04). Neurons and vessels were also immunopositive for TDO2, but there were no significant differences in labeling of these structures between the two groups. These results add to the evidence that kynurenine pathway changes might be involved in the pathogenesis of schizophrenia and the schizophrenia-like psychoses of other disorders.

Original languageEnglish (US)
Pages (from-to)618-629
Number of pages12
JournalNeurobiology of Disease
Volume15
Issue number3
DOIs
StatePublished - Apr 1 2004

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Keywords

  • Human
  • INDO
  • Immunohistochemistry
  • Indoleamine dioxygenase
  • Protein
  • Psychosis
  • RT-PCR
  • TDO2
  • Tryptophan pyrrolase
  • mRNA

ASJC Scopus subject areas

  • Neurology

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