Expression of the high affinity IgE receptor by neutrophils of individuals with allergic asthma is both minimal and insensitive to regulation by serum IgE

Juanita Mora, Emily K. Riggs, Jun Fu, Donald Macglashan, Susan A. Fox, Byung Yu, Mary C. Tobin, Larry L. Thomas

Research output: Contribution to journalArticle

Abstract

We evaluated the hypothesis that serum IgE regulates neutrophil FcεRI expression in the same manner as described for other FcεRI+ cells. FcεRI expression by neutrophils of 40 asthma subjects and 20 control subjects did not correlate with serum IgE levels, whereas FcεRI expression by basophils of the same subjects showed a highly significant correlation. The level of FcεRI expression by neutrophils of both asthma and control subjects was approximately 1% of that for basophil FcεRI expression. IgE+ neutrophils were minimally detectable, and FcεRI α-subunit was not detected in Western blots of neutrophil membranes and cytosol. The neutrophil FcεRI did not support anti-IgE stimulated superoxide release or IgE-induced increase in neutrophil survival. We conclude that FcεRI expression by neutrophils of both asthma patients and control individuals is minimal at best and that, if present, neutrophil FcεRI expression, unlike that of other human FcεRI+ cells, is not regulated by serum IgE.

Original languageEnglish (US)
Pages (from-to)132-140
Number of pages9
JournalClinical Immunology
Volume132
Issue number1
DOIs
StatePublished - Jul 2009

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IgE Receptors
Immunoglobulin E
Neutrophils
Asthma
Serum
Basophils
Superoxides
Cytosol
Western Blotting
Membranes

Keywords

  • Allergic asthma
  • Basophils
  • Fc receptors
  • High affinity IgE receptor
  • IgE antibody
  • Neutrophils

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Expression of the high affinity IgE receptor by neutrophils of individuals with allergic asthma is both minimal and insensitive to regulation by serum IgE. / Mora, Juanita; Riggs, Emily K.; Fu, Jun; Macglashan, Donald; Fox, Susan A.; Yu, Byung; Tobin, Mary C.; Thomas, Larry L.

In: Clinical Immunology, Vol. 132, No. 1, 07.2009, p. 132-140.

Research output: Contribution to journalArticle

Mora, Juanita ; Riggs, Emily K. ; Fu, Jun ; Macglashan, Donald ; Fox, Susan A. ; Yu, Byung ; Tobin, Mary C. ; Thomas, Larry L. / Expression of the high affinity IgE receptor by neutrophils of individuals with allergic asthma is both minimal and insensitive to regulation by serum IgE. In: Clinical Immunology. 2009 ; Vol. 132, No. 1. pp. 132-140.
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AU - Yu, Byung

AU - Tobin, Mary C.

AU - Thomas, Larry L.

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