Expression of the amyloid precursor protein gene in mouse oocytes and embryos

Shannon Fisher, John D. Gearhart, Mary L. Oster-Granite

Research output: Contribution to journalArticle

Abstract

The amyloid precursor protein (APP) is thought to be processed aberrantly to yield the major constituent of the amyloid plaques observed in the brains of patients with Alzheimer disease and Down syndrome. However, the gene encoding APP is expressed widely in normal human tissues and in adult and fetal mouse tissues and is alternately spliced in a tissue-specific pattern in the adult. There is evidence that APP may function as a growth factor and as a mediator of cell adhesion and in these roles could be important in morphogenesis. As a step toward determining the role of APP in development and in determining how the adult pattern of tissuespecific splicing is established, we have used reverse transcription and the polymerase chain reaction to demonstrate APP expression in mouse oocytes, preimplantation embryos, and postimplantation embryonic stages to the late embryonic period. All three splicing forms described in mouse were present at each stage, although there were changes in the ratios of the splicing forms at different stages. Screens for APP clones in embryonic cDNA libraries from the egg cylinder stage and the early somite stage were used to confirm the results of the polymerase chain reaction, and APP clone abundance was found to increase 10-fold between these two stages.

Original languageEnglish (US)
Pages (from-to)1779-1782
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number5
StatePublished - 1991

Fingerprint

Amyloid beta-Protein Precursor
Oocytes
Embryonic Structures
Genes
Alzheimer Disease
Clone Cells
Polymerase Chain Reaction
Somites
Amyloid Plaques
Blastocyst
Down Syndrome
Gene Library
Morphogenesis
Cell Adhesion
Reverse Transcription
Ovum
Intercellular Signaling Peptides and Proteins
Fetus
Brain

Keywords

  • β-amyloid
  • Alzheimer disease
  • Down syndrome
  • Polymerase chain reaction

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Expression of the amyloid precursor protein gene in mouse oocytes and embryos. / Fisher, Shannon; Gearhart, John D.; Oster-Granite, Mary L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, No. 5, 1991, p. 1779-1782.

Research output: Contribution to journalArticle

Fisher, Shannon ; Gearhart, John D. ; Oster-Granite, Mary L. / Expression of the amyloid precursor protein gene in mouse oocytes and embryos. In: Proceedings of the National Academy of Sciences of the United States of America. 1991 ; Vol. 88, No. 5. pp. 1779-1782.
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