Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum

Fadi G. Lakkis; Eddie N. Cruet; George M. Nassar; Kamal F. Badr; David W. Pascual

doi:10.1006/bbrc.1997.6832

Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum

Fadi G. Lakkis, Eddie N. Cruet, George M. Nassar, Kamal F. Badr, David W. Pascual

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in human peripheral blood monocytes. Antiserum generated by immunizing a rabbit with recombinant bacterial rat IL-13 specifically inhibited TF-1 proliferation induced by baculoviral rat IL-13 but did not neutralize human IL-13 mitogenic activity. Western blotting with anti-rat IL-13 serum revealed a ~12 kD protein band in supernatants of insect cells infected with recombinant baculovirus carrying the rat IL-13 cDNA. The availability of recombinant rat IL-13 and rat IL-13 antibodies should facilitate studying the role of IL-13 in rat models of human inflammatory disorders.

Original language	English (US)
Pages (from-to)	529-532
Number of pages	4
Journal	Biochemical and Biophysical Research Communications
Volume	235
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1997.6832
State	Published - Jun 27 1997
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1006/bbrc.1997.6832

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title = "Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum",

abstract = "Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in human peripheral blood monocytes. Antiserum generated by immunizing a rabbit with recombinant bacterial rat IL-13 specifically inhibited TF-1 proliferation induced by baculoviral rat IL-13 but did not neutralize human IL-13 mitogenic activity. Western blotting with anti-rat IL-13 serum revealed a ~12 kD protein band in supernatants of insect cells infected with recombinant baculovirus carrying the rat IL-13 cDNA. The availability of recombinant rat IL-13 and rat IL-13 antibodies should facilitate studying the role of IL-13 in rat models of human inflammatory disorders.",

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AU - Cruet, Eddie N.

AU - Nassar, George M.

AU - Badr, Kamal F.

AU - Pascual, David W.

PY - 1997/6/27

Y1 - 1997/6/27

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Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum

Abstract

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