Expression of recombinant rat interleukin-13 (IL-13) and generation of a neutralizing rat IL-13 antiserum

Fadi G. Lakkis, Eddie N. Cruet, George M. Nassar, Kamal F. Badr, David W. Pascual

Research output: Contribution to journalArticlepeer-review

Abstract

Using baculoviral and bacterial systems, we expressed biologically active recombinant rat IL-13 and generated neutralizing rat IL-13 antiserum. Recombinant rat IL-13 produced by baculovirus-infected insect cells stimulated proliferation of TF-1 premyeloid cell line and induced expression of 15-lipoxygenase mRNA in human peripheral blood monocytes. Antiserum generated by immunizing a rabbit with recombinant bacterial rat IL-13 specifically inhibited TF-1 proliferation induced by baculoviral rat IL-13 but did not neutralize human IL-13 mitogenic activity. Western blotting with anti-rat IL-13 serum revealed a ~12 kD protein band in supernatants of insect cells infected with recombinant baculovirus carrying the rat IL-13 cDNA. The availability of recombinant rat IL-13 and rat IL-13 antibodies should facilitate studying the role of IL-13 in rat models of human inflammatory disorders.

Original languageEnglish (US)
Pages (from-to)529-532
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume235
Issue number3
DOIs
StatePublished - Jun 27 1997
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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