Expression of programmed death 1 ligands by murine T cells and APC

Tomohide Yamazaki, Hisaya Akiba, Hideyuki Iwai, Hironori Matsuda, Mami Aoki, Yuka Tanno, Tahiro Shin, Haruo Tsuchiya, Andrew Mark Pardoll, Ko Okumura, Miyuki Azuma, Hideo Yagita

Research output: Contribution to journalArticle

Abstract

Programmed death 1 (PD-1) is a new member of the CD28/CTLA-4 family, which has been implicated in the maintenance of peripheral tolerance. Two ligands for PD-1, namely, B7-H1 (PD-L1) and B7-DC (PD-L2), have recently been identified as new members of the B7 family but their expression at the protein level remains largely unknown. To characterize the expression of B7-H1 and B7-DC, we newly generated an anti-mouse B7-H1 mAb (MIH6) and an anti-mouse B7-DC mAb (TY25). MIH6 and TY25 immunoprecipitated a single molecule of 43 and 42 kDa from the lysate of B7-H1 and B7-DC transfectants, respectively. Flow cytometric analysis revealed that B7-H1 was broadly expressed on the surface of mouse tumor cell lines while the expression of B7-DC was rather restricted. PD-1 was expressed on anti-CD3-stimulated T cells and anti-IgM plus anti-CD40-stimulated B cells at high levels but was undetectable on activated macrophages or DCs. B7-H1 was constitutively expressed on freshly isolated splenic T cells, B cells, macrophages, and dendritic cells (DCs), and up-regulated on T cells by anti-CD3 stimulation on macrophages by LPS, IFN-γ, GM-CSF, or IL-4, and on DCs by IFN-γ, GM-CSF, or IL-4. In contrast, B7-DC expression was only inducible on macrophages and DCs upon stimulation with IFN-γ, GM-CSF, or IL-4. The inducible expression of PD-1 ligands on both T cells and APCs may suggest new paradigms of PD-1-mediated immune regulation.

Original languageEnglish (US)
Pages (from-to)5538-5545
Number of pages8
JournalJournal of Immunology
Volume169
Issue number10
StatePublished - Nov 15 2002
Externally publishedYes

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Dendritic Cells
Ligands
T-Lymphocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Macrophages
Interleukin-4
B-Lymphocytes
Peripheral Tolerance
Tumor Cell Line
Maintenance

ASJC Scopus subject areas

  • Immunology

Cite this

Yamazaki, T., Akiba, H., Iwai, H., Matsuda, H., Aoki, M., Tanno, Y., ... Yagita, H. (2002). Expression of programmed death 1 ligands by murine T cells and APC. Journal of Immunology, 169(10), 5538-5545.

Expression of programmed death 1 ligands by murine T cells and APC. / Yamazaki, Tomohide; Akiba, Hisaya; Iwai, Hideyuki; Matsuda, Hironori; Aoki, Mami; Tanno, Yuka; Shin, Tahiro; Tsuchiya, Haruo; Pardoll, Andrew Mark; Okumura, Ko; Azuma, Miyuki; Yagita, Hideo.

In: Journal of Immunology, Vol. 169, No. 10, 15.11.2002, p. 5538-5545.

Research output: Contribution to journalArticle

Yamazaki, T, Akiba, H, Iwai, H, Matsuda, H, Aoki, M, Tanno, Y, Shin, T, Tsuchiya, H, Pardoll, AM, Okumura, K, Azuma, M & Yagita, H 2002, 'Expression of programmed death 1 ligands by murine T cells and APC', Journal of Immunology, vol. 169, no. 10, pp. 5538-5545.
Yamazaki T, Akiba H, Iwai H, Matsuda H, Aoki M, Tanno Y et al. Expression of programmed death 1 ligands by murine T cells and APC. Journal of Immunology. 2002 Nov 15;169(10):5538-5545.
Yamazaki, Tomohide ; Akiba, Hisaya ; Iwai, Hideyuki ; Matsuda, Hironori ; Aoki, Mami ; Tanno, Yuka ; Shin, Tahiro ; Tsuchiya, Haruo ; Pardoll, Andrew Mark ; Okumura, Ko ; Azuma, Miyuki ; Yagita, Hideo. / Expression of programmed death 1 ligands by murine T cells and APC. In: Journal of Immunology. 2002 ; Vol. 169, No. 10. pp. 5538-5545.
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AU - Tanno, Yuka

AU - Shin, Tahiro

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AU - Yagita, Hideo

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