Expression of programmed death 1 ligands by murine T cells and APC

Tomohide Yamazaki, Hisaya Akiba, Hideyuki Iwai, Hironori Matsuda, Mami Aoki, Yuka Tanno, Tahiro Shin, Haruo Tsuchiya, Drew M. Pardoll, Ko Okumura, Miyuki Azuma, Hideo Yagita

Research output: Contribution to journalArticle

Abstract

Programmed death 1 (PD-1) is a new member of the CD28/CTLA-4 family, which has been implicated in the maintenance of peripheral tolerance. Two ligands for PD-1, namely, B7-H1 (PD-L1) and B7-DC (PD-L2), have recently been identified as new members of the B7 family but their expression at the protein level remains largely unknown. To characterize the expression of B7-H1 and B7-DC, we newly generated an anti-mouse B7-H1 mAb (MIH6) and an anti-mouse B7-DC mAb (TY25). MIH6 and TY25 immunoprecipitated a single molecule of 43 and 42 kDa from the lysate of B7-H1 and B7-DC transfectants, respectively. Flow cytometric analysis revealed that B7-H1 was broadly expressed on the surface of mouse tumor cell lines while the expression of B7-DC was rather restricted. PD-1 was expressed on anti-CD3-stimulated T cells and anti-IgM plus anti-CD40-stimulated B cells at high levels but was undetectable on activated macrophages or DCs. B7-H1 was constitutively expressed on freshly isolated splenic T cells, B cells, macrophages, and dendritic cells (DCs), and up-regulated on T cells by anti-CD3 stimulation on macrophages by LPS, IFN-γ, GM-CSF, or IL-4, and on DCs by IFN-γ, GM-CSF, or IL-4. In contrast, B7-DC expression was only inducible on macrophages and DCs upon stimulation with IFN-γ, GM-CSF, or IL-4. The inducible expression of PD-1 ligands on both T cells and APCs may suggest new paradigms of PD-1-mediated immune regulation.

Original languageEnglish (US)
Pages (from-to)5538-5545
Number of pages8
JournalJournal of Immunology
Volume169
Issue number10
DOIs
StatePublished - Nov 15 2002

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Yamazaki, T., Akiba, H., Iwai, H., Matsuda, H., Aoki, M., Tanno, Y., Shin, T., Tsuchiya, H., Pardoll, D. M., Okumura, K., Azuma, M., & Yagita, H. (2002). Expression of programmed death 1 ligands by murine T cells and APC. Journal of Immunology, 169(10), 5538-5545. https://doi.org/10.4049/jimmunol.169.10.5538