TY - JOUR
T1 - Expression of precore and core proteins of hepatitis B virus in human hepatoma cell line.
AU - Liu, Ding Xie
AU - Dai, Lin
AU - Luo, Kang Xian
PY - 2002
Y1 - 2002
N2 - To further investigate the functions of proteins encoded by C open reading frame of hepatitis B virus (HBV) in HBV infectious cycle, cell lines expressing these proteins were established. The DNA fragments encoding 25 000, 22 000 precore-core and 21 000 core protein respectively were amplified from plasmid pCP10 which contained HBV genome, and inserted into a eukaryotic expression vector pcDNA-3, and then transfected into hepatoma cell line HepG2. The transformants were selected with neomycin, and three isolated colonies expressing precore or core proteins were obtained stably, which were demonstrated by immunohistochemistry and Western blotting analysis.
AB - To further investigate the functions of proteins encoded by C open reading frame of hepatitis B virus (HBV) in HBV infectious cycle, cell lines expressing these proteins were established. The DNA fragments encoding 25 000, 22 000 precore-core and 21 000 core protein respectively were amplified from plasmid pCP10 which contained HBV genome, and inserted into a eukaryotic expression vector pcDNA-3, and then transfected into hepatoma cell line HepG2. The transformants were selected with neomycin, and three isolated colonies expressing precore or core proteins were obtained stably, which were demonstrated by immunohistochemistry and Western blotting analysis.
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M3 - Article
C2 - 12390822
AN - SCOPUS:0036481579
SN - 1000-2588
VL - 22
SP - 174
EP - 176
JO - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
JF - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
IS - 2
ER -