Expression of precore and core proteins of hepatitis B virus in human hepatoma cell line.

Ding Xie Liu, Lin Dai, Kang Xian Luo

Research output: Contribution to journalArticle

Abstract

To further investigate the functions of proteins encoded by C open reading frame of hepatitis B virus (HBV) in HBV infectious cycle, cell lines expressing these proteins were established. The DNA fragments encoding 25 000, 22 000 precore-core and 21 000 core protein respectively were amplified from plasmid pCP10 which contained HBV genome, and inserted into a eukaryotic expression vector pcDNA-3, and then transfected into hepatoma cell line HepG2. The transformants were selected with neomycin, and three isolated colonies expressing precore or core proteins were obtained stably, which were demonstrated by immunohistochemistry and Western blotting analysis.

Original languageEnglish (US)
Pages (from-to)174-176
Number of pages3
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume22
Issue number2
Publication statusPublished - 2002
Externally publishedYes

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