Expression of Pdx-1 in human gastric metaplasia and gastric adenocarcinoma

Charles M. Leys, Sachiyo Nomura, Erin Rudzinski, Michio Kaminishi, Elizabeth Montgomery, Mary Kay Washington, James R. Goldenring

Research output: Contribution to journalArticlepeer-review

Abstract

Metaplastic lineages represent critical putative preneoplastic precursors for gastrointestinal metaplasia. Two metaplastic processes are associated with gastric cancer: intestinal metaplasia (the presence of intestinal goblet cell containing lineages in the stomach) and spasmolytic polypeptide-expressing metaplasia (SPEM; antralization of the gastric fundus). The transcription factor Pdx-1 is expressed in the adult pancreatic islet cells as well as the gastric antrum and duodenum. We have previously noted the increase in Pdx-1 expression in models of TGFα overexpression in mice but not in other models of SPEM in rodents. We have therefore sought to examine the presence of Pdx-1 expression in gastric metaplasias and gastric adenocarcinoma in humans. Tissue microarrays containing gastric cancers from the fundus and antrum and samples of SPEM and intestinal metaplasia were immunostained for Pdx-1. Nuclear Pdx-1 expression was observed in only 50% of antral-derived cancers and was present in 40% of fundic tumors. Pdx-1 expression did not correlate with clinical outcome. Although SPEM lineages did not show any staining for Pdx-1, intestinal metaplasia showed strong nuclear staining for Pdx-1. Thus, Pdx-1 expression is not associated with antralizing metaplasia (SPEM) but is associated with intestinal metaplasia. Given the pattern of normal Pdx-1 expression in the duodenum, goblet cell metaplasia in the stomach may reflect the adoption of a duodenal lineage paradigm.

Original languageEnglish (US)
Pages (from-to)1162-1168
Number of pages7
JournalHuman pathology
Volume37
Issue number9
DOIs
StatePublished - Sep 2006

Keywords

  • Antrum
  • Biomarkers
  • Gastric cancer
  • Intestinal metaplasia
  • Pdx-1
  • SPEM
  • TFF2

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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