Expression of ocular albinism 1 (OA1), 3, 4- dihydroxy- L-phenylalanine (DOPA) receptor, in both neuronal and non-neuronal organs

Nobuhiko Fukuda, Saki Naito, Daiki Masukawa, Moemi Kaneda, Hiroshi Miyamoto, Takaya Abe, Yui Yamashita, Itaru Endo, Fumio Nakamura, Yoshio Goshima

Research output: Contribution to journalArticle

Abstract

Oa1 is the casual gene for ocular albinism-1 in humans. The gene product OA1, alternatively designated as GPR143, belongs to G-protein coupled receptors.It has been reported that OA1 is a specific receptorfor3, 4-dihydroxy-L-phenylalanine (DOPA) in retinal pigmental epithelium where DOPA facilitates the pigmentation via OA1 stimulation.We have recently shown that OA1 mediates DOPA-induced depressor response in rat nucleus tractus solitarii. However,the distribution and function of OA1 in other regions are largely unknown.We have generated oa1 knockout mice and examined OA1 expression in both neuronal and non-neuronal tissues by immunohistochemical analyses using anti-mouse OA1 monoclonal antibodies. In the telencephalon, OA1 was expressed in cerebral cortex and hippocampus. Predominant expression of OA1 was observed in the pyramidal neurons in these regions.OA1 was also expressed in habenular nucleus, hypothalamus, substantia nigra, and medulla oblongata.The expression of OA1 in the nucleus tractus solitarii of medulla oblongata may support the reduction of blood pressure by the microinjection of DOPA into this region. Outside of the nervous system, OA1 was expressed in heart, lung, liver, kidney and spleen. Abundant expression was observed in the renal tubules and the splenic capsules.These peripheral regions are innervated by numerous sympathetic nerve endings. In addition, substantia nigra contains a large population of dopaminergic neurons.Thus,the immunohistochemical analyses suggest that OA1 may modulate the monoaminergic functions in both peripheral and central nervous systems.

Original languageEnglish (US)
Pages (from-to)62-74
Number of pages13
JournalBrain Research
Volume1602
Issue numberC
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • DOPA
  • GPR143
  • Immunohistochemistry
  • Knockout mice
  • OA1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology
  • Medicine(all)

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  • Cite this

    Fukuda, N., Naito, S., Masukawa, D., Kaneda, M., Miyamoto, H., Abe, T., Yamashita, Y., Endo, I., Nakamura, F., & Goshima, Y. (2015). Expression of ocular albinism 1 (OA1), 3, 4- dihydroxy- L-phenylalanine (DOPA) receptor, in both neuronal and non-neuronal organs. Brain Research, 1602(C), 62-74. https://doi.org/10.1016/j.brainres.2015.01.020