Expression of neu antigen in normal lactating and prepartum mammary epithelial cells

Barry M. Kacinski, Josephine Nguyen, Darryl Carter

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the expression of the neu protein antigen in non-neoplastic, mammary epithelial cells of prepartum and actively lactating women. Methods: Formalin-fixed, paraffin-embedded sections from breast biopsies obtained from four prepartum and seven postpartum, lactating women were stained for neu antigen with one commercially available monoclonal antibody (Ab-3), one commercially available polyclonal antibody (Ab-1), and another independently prepared polyclonal antibody (21N). Antigen competition experiments were carried out alongside as negative controls, and two strongly neu-positive breast carcinomas were included as positive controls. Results: Membranes and cytoplasmic elements of two of the four prepartum and four of the seven lactating specimens stained as strongly or nearly as strongly for neu antigen as did those of the breast carcinomas. Conclusion: Our results demonstrate the expression of neu antigen by mammary epithelial cells just before and during lactation and suggest a possible role for this receptor in the lactational differentiation of the normal female breast.

Original languageEnglish (US)
Pages (from-to)51-56
Number of pages6
JournalJournal of the Society for Gynecologic Investigation
Volume2
Issue number1
StatePublished - Jan 1995
Externally publishedYes

Fingerprint

Breast
Epithelial Cells
Antigens
Breast Neoplasms
Antibodies
Lactation
Paraffin
Postpartum Period
Formaldehyde
Monoclonal Antibodies
Cell Membrane
Biopsy
Proteins

Keywords

  • breast
  • HER-2/neu
  • immunohistochemistry
  • lactation
  • pregnancy

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Expression of neu antigen in normal lactating and prepartum mammary epithelial cells. / Kacinski, Barry M.; Nguyen, Josephine; Carter, Darryl.

In: Journal of the Society for Gynecologic Investigation, Vol. 2, No. 1, 01.1995, p. 51-56.

Research output: Contribution to journalArticle

Kacinski, Barry M. ; Nguyen, Josephine ; Carter, Darryl. / Expression of neu antigen in normal lactating and prepartum mammary epithelial cells. In: Journal of the Society for Gynecologic Investigation. 1995 ; Vol. 2, No. 1. pp. 51-56.
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