TY - JOUR
T1 - Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage
AU - Yano, Kazuhiko
AU - Komaki-Yasuda, Kanako
AU - Kobayashi, Tamaki
AU - Takemae, Hitoshi
AU - Kita, Kiyoshi
AU - Kano, Shigeyuki
AU - Kawazu, Shin Ichiro
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas (2) (15019127 and 16017318 to S.K., and 14021014 to K.K.) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, and a Grant for Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency (to S.K.). Sequence data for P. falciparum chromosomes were obtained from the Plasmodium Genome Consortium PlasmoDB (PlasmoDB; http://PlasmoDB.org ).
PY - 2005/3
Y1 - 2005/3
N2 - mRNA and protein expression profiles for three peroxiredoxins (PfTPx-1, PfTPx-2 and Pf1-Cys-Prx) and a thioredoxin (PfTrx-1) of Plasmodium falciparum during the erythrocytic stage were examined by real-time quantitative reverse transcription-PCR (RT-PCR), Western blotting and confocal laser scanning microscopy. PfTPx-1 was expressed constitutively in the parasite cytoplasm throughout the erythrocytic stage, suggesting a housekeeping role of this enzyme for control of intercellular reactive oxygen species (ROS) in the parasite. Pf1-Cys-Prx showed elevated expression during the trophozoite and early schizont stages in the parasite cytoplasm, and this profile suggested that this peroxiredoxin (Prx) detoxifies metabolism-derived ROS such as those released from heme iron. The other 2-Cys Prx, PfTPx-2, was detected in mitochondria and was expressed in both the trophozoite and schizont stages. Detection of the Prx in mitochondria is consistent with recent reports of the existence of a respiratory chain, which produces ROS, in the mitochondria of P. falciparum. PfTrx-1 showed elevated expression during the trophozoite and schizont stages in the parasite cytoplasm. Finally, expression of these antioxidant protein genes is most likely regulated at the transcriptional level because their mRNA and protein expression profiles overlapped.
AB - mRNA and protein expression profiles for three peroxiredoxins (PfTPx-1, PfTPx-2 and Pf1-Cys-Prx) and a thioredoxin (PfTrx-1) of Plasmodium falciparum during the erythrocytic stage were examined by real-time quantitative reverse transcription-PCR (RT-PCR), Western blotting and confocal laser scanning microscopy. PfTPx-1 was expressed constitutively in the parasite cytoplasm throughout the erythrocytic stage, suggesting a housekeeping role of this enzyme for control of intercellular reactive oxygen species (ROS) in the parasite. Pf1-Cys-Prx showed elevated expression during the trophozoite and early schizont stages in the parasite cytoplasm, and this profile suggested that this peroxiredoxin (Prx) detoxifies metabolism-derived ROS such as those released from heme iron. The other 2-Cys Prx, PfTPx-2, was detected in mitochondria and was expressed in both the trophozoite and schizont stages. Detection of the Prx in mitochondria is consistent with recent reports of the existence of a respiratory chain, which produces ROS, in the mitochondria of P. falciparum. PfTrx-1 showed elevated expression during the trophozoite and schizont stages in the parasite cytoplasm. Finally, expression of these antioxidant protein genes is most likely regulated at the transcriptional level because their mRNA and protein expression profiles overlapped.
KW - Antioxidant
KW - Malaria
KW - Peroxiredoxin
KW - Plasmodium falciparum
KW - Thioredoxin
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U2 - 10.1016/j.parint.2004.08.005
DO - 10.1016/j.parint.2004.08.005
M3 - Article
C2 - 15710548
AN - SCOPUS:13844267219
SN - 1383-5769
VL - 54
SP - 35
EP - 41
JO - Parasitology International
JF - Parasitology International
IS - 1
ER -