Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage

Kazuhiko Yano, Kanako Komaki-Yasuda, Tamaki Kobayashi, Hitoshi Takemae, Kiyoshi Kita, Shigeyuki Kano, Shin Ichiro Kawazu

Research output: Contribution to journalArticle

Abstract

mRNA and protein expression profiles for three peroxiredoxins (PfTPx-1, PfTPx-2 and Pf1-Cys-Prx) and a thioredoxin (PfTrx-1) of Plasmodium falciparum during the erythrocytic stage were examined by real-time quantitative reverse transcription-PCR (RT-PCR), Western blotting and confocal laser scanning microscopy. PfTPx-1 was expressed constitutively in the parasite cytoplasm throughout the erythrocytic stage, suggesting a housekeeping role of this enzyme for control of intercellular reactive oxygen species (ROS) in the parasite. Pf1-Cys-Prx showed elevated expression during the trophozoite and early schizont stages in the parasite cytoplasm, and this profile suggested that this peroxiredoxin (Prx) detoxifies metabolism-derived ROS such as those released from heme iron. The other 2-Cys Prx, PfTPx-2, was detected in mitochondria and was expressed in both the trophozoite and schizont stages. Detection of the Prx in mitochondria is consistent with recent reports of the existence of a respiratory chain, which produces ROS, in the mitochondria of P. falciparum. PfTrx-1 showed elevated expression during the trophozoite and schizont stages in the parasite cytoplasm. Finally, expression of these antioxidant protein genes is most likely regulated at the transcriptional level because their mRNA and protein expression profiles overlapped.

Original languageEnglish (US)
Pages (from-to)35-41
Number of pages7
JournalParasitology International
Volume54
Issue number1
DOIs
StatePublished - Mar 2005
Externally publishedYes

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Peroxiredoxins
Plasmodium falciparum
Schizonts
Trophozoites
Messenger RNA
Parasites
Reactive Oxygen Species
Mitochondria
Cytoplasm
Proteins
Housekeeping
Thioredoxins
Electron Transport
Heme
Confocal Microscopy
Reverse Transcription
Iron
Antioxidants
Western Blotting
Polymerase Chain Reaction

Keywords

  • Antioxidant
  • Malaria
  • Peroxiredoxin
  • Plasmodium falciparum
  • Thioredoxin

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage. / Yano, Kazuhiko; Komaki-Yasuda, Kanako; Kobayashi, Tamaki; Takemae, Hitoshi; Kita, Kiyoshi; Kano, Shigeyuki; Kawazu, Shin Ichiro.

In: Parasitology International, Vol. 54, No. 1, 03.2005, p. 35-41.

Research output: Contribution to journalArticle

Yano, Kazuhiko ; Komaki-Yasuda, Kanako ; Kobayashi, Tamaki ; Takemae, Hitoshi ; Kita, Kiyoshi ; Kano, Shigeyuki ; Kawazu, Shin Ichiro. / Expression of mRNAs and proteins for peroxiredoxins in Plasmodium falciparum erythrocytic stage. In: Parasitology International. 2005 ; Vol. 54, No. 1. pp. 35-41.
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