Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger Mouse

Masahito Kamanaka, Sean T. Kim, Yisong Y. Wan, Fayyaz S. Sutterwala, Maria Lara-Tejero, Jorge E. Galán, Ed Harhaj, Richard A. Flavell

Research output: Contribution to journalArticlepeer-review

Abstract

To identify interleukin-10 (IL-10)-producing cells in vivo, we generated a knockin mouse where an internal ribosome entry site (IRES) green fluorescence protein (GFP) element was inserted immediately before the polyadenylation site of the IL-10 gene. GFP fluorescence in cells from these mice was found to correlate positively with IL-10 protein expression. With this model, we found that after multiple T cell receptor (TCR) stimulations, strong expression of IL-10 was produced specifically by intraepithelial lymphocytes (IEL) in the small intestine and colonic lamina propria lymphocytes (cLPL). We found that anti-CD3 treatment induces T regulatory cell 1 (Tr1)-like cells in small intestinal IEL (sIEL) and led to the accumulation of naturally occurring regulatory T (nTreg) cells in colonic LPL (cLPL). These findings highlight the intestine as a unique site for induction of IL-10-producing T cells, which play a critical role in the regulation of inflammation in the gut.

Original languageEnglish (US)
Pages (from-to)941-952
Number of pages12
JournalImmunity
Volume25
Issue number6
DOIs
StatePublished - Dec 2006

Keywords

  • CELLIMMUNO
  • HUMDISEASE
  • MOLIMMUNO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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