Expression of Id1 results in apoptosis of cardiac myocytes through a redox-dependent mechanism

Koichi Tanaka, John B. Pracyk, Kazuyo Takeda, Zu Xi Yu, Victor J. Ferrans, Shailesh S. Deshpande, Michitaka Ozaki, Paul M. Hwang, Charles J. Lowenstein, Kaikobad Irani, Toren Finkel

Research output: Contribution to journalArticle

Abstract

We have constructed a recombinant adenovirus (Ad.Id1) that allows for efficient expression of the helix-loop-helix protein Id1. After infection with Ad.Id1, neonatal cardiac myocytes display a significant reduction in viability, which was proportional to the level of Id1 expression. A similar effect was observed in adult myocytes. Morphological and biochemical assays demonstrated that Id1 expression resulted in myocyte apoptosis. In contrast, expression of Id1 in endothelial cells, vascular smooth muscle cells, or fibroblasts did not affect the viability of these cells. Along with the induction of apoptosis, the expression of Id1 in neonatal cardiac myocytes resulted in an increase in the level of intracellular reactive oxygen species. The source of these reactive oxygen species appears to be the mitochondria. Reducing the ambient oxygen concentration or treatment with a cell-permeant H2O2 scavenger prevented Id1-stimulated apoptosis in cardiac myocytes. These results suggest that the expression of Id1 leads to the induction of apoptosis in cardiac myocytes through a redox-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)25922-25928
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number40
DOIs
StatePublished - Oct 2 1998

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Cardiac Myocytes
Oxidation-Reduction
Apoptosis
Muscle Cells
Reactive Oxygen Species
Cells
Mitochondria
Endothelial cells
Fibroblasts
Vascular Smooth Muscle
Adenoviridae
Smooth Muscle Myocytes
Muscle
Assays
Cell Survival
Endothelial Cells
Oxygen
Infection
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tanaka, K., Pracyk, J. B., Takeda, K., Yu, Z. X., Ferrans, V. J., Deshpande, S. S., ... Finkel, T. (1998). Expression of Id1 results in apoptosis of cardiac myocytes through a redox-dependent mechanism. Journal of Biological Chemistry, 273(40), 25922-25928. https://doi.org/10.1074/jbc.273.40.25922

Expression of Id1 results in apoptosis of cardiac myocytes through a redox-dependent mechanism. / Tanaka, Koichi; Pracyk, John B.; Takeda, Kazuyo; Yu, Zu Xi; Ferrans, Victor J.; Deshpande, Shailesh S.; Ozaki, Michitaka; Hwang, Paul M.; Lowenstein, Charles J.; Irani, Kaikobad; Finkel, Toren.

In: Journal of Biological Chemistry, Vol. 273, No. 40, 02.10.1998, p. 25922-25928.

Research output: Contribution to journalArticle

Tanaka, K, Pracyk, JB, Takeda, K, Yu, ZX, Ferrans, VJ, Deshpande, SS, Ozaki, M, Hwang, PM, Lowenstein, CJ, Irani, K & Finkel, T 1998, 'Expression of Id1 results in apoptosis of cardiac myocytes through a redox-dependent mechanism', Journal of Biological Chemistry, vol. 273, no. 40, pp. 25922-25928. https://doi.org/10.1074/jbc.273.40.25922
Tanaka, Koichi ; Pracyk, John B. ; Takeda, Kazuyo ; Yu, Zu Xi ; Ferrans, Victor J. ; Deshpande, Shailesh S. ; Ozaki, Michitaka ; Hwang, Paul M. ; Lowenstein, Charles J. ; Irani, Kaikobad ; Finkel, Toren. / Expression of Id1 results in apoptosis of cardiac myocytes through a redox-dependent mechanism. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 40. pp. 25922-25928.
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