Expression of ICAM-1 in airway epithelium after acute ozone exposure in the mouse

N. Takahashi, X. Y. Yu, B. H. Schofield, S. R. Kleeberger, A. L. Scott, S. Hasegawa, E. W. Spannhake

Research output: Contribution to journalArticle

Abstract

We investigated the time course and regional distribution of the expression of intercellular adhesion molecule-1 (ICAM-1) on airway epithelial cells and the polymorphonuclear leukocyte (PMN) inflammatory response in the lung after acute exposure to ozone (O3). C57BL/6J mice were exposed to air or 2 ppm O3 for 3 h and killed immediately or 3, 6, 9, or 21 h after exposure. Expression of ICAM-1 was examined by immunohistochemical staining of frozen sections. PMN influx was evaluated by lavage and by histochemical staining of myeloperoxidase (MPO) and measurement of tissue MPO activity. ICAM-1 expression exhibited regional selectivity and temporal patterns that were unique to each region. Upregulation of ICAM-1 expression on the epithelial cells in the trachea, and to a lesser extent in the lobar and segmental bronchi, was observed 3-9 h after exposure and remained present at 21 h. Enhanced ICAM-1 expression in bronchioles and terminal bronchiole/alveolar duct regions was evident earlier (immediately to 3 h after exposure) but returned to baseline levels by 21 and 9 h, respectively. Maximal ICAM-1 expression and PMN influx in the lung parenchyma were concurrently observed at 3 h, followed by transepithelial migration of PMNs to the airway lumen. These results demonstrate regional variations in airway inflammatory activity and are supportive of the notion that upregulation of ICAM-1 on the airway epithelium may play a role in local regulation of PMN influx to the airways after acute O3 exposure.

Original languageEnglish (US)
Pages (from-to)1753-1761
Number of pages9
JournalJournal of applied physiology
Volume79
Issue number5
DOIs
StatePublished - Jan 1 1995

Keywords

  • adhesion molecule
  • immunohistochemistry
  • inflammation
  • intercellular adhesion molecule-1
  • myeloperoxidase
  • neutrophils

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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