Expression of hypoxia-inducible factor-1α and -2α in whole-mount prostate histology: Relation with dynamic contrast-enhanced MRI and Gleason score

Alie Borren, Greetje Groenendaal, Petra Van Der Groep, Maaike R. Moman, Arto E. Boeken Kruger, Uulke A. Van Der Heide, Trudy N. Jonges, Paul J. Van Diest, Marco Van Vulpen, Marielle E P Philippens

Research output: Contribution to journalArticle

Abstract

The aim of this study was to investigate the association between the immunohistochemical expression of hypoxia-inducible factor (HIF)-1α and HIF-2α and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) parameters Ktrans and kep in prostate cancer. Therefore, 15 patients with biopsy-confirmed prostate cancer underwent a pre-operative 3T DCE-MRI scan. Immunohistochemical analysis of HIF-1α and HIF-2α, and of CD31 for microvessel density (MVD) was performed. Tumor areas were delineated on whole-mount histopathological sections. Nuclear HIF expression was correlated with the quantitative DCE-MRI parameters Ktrans and kep, MVD and Gleason score. HIF expression was highly heterogeneous within tumors and between patients. Pronounced expression of HIF-2α was present, while HIF-1α expression was more limited. Larger tumors showed higher HIF-2α expression (P=0.041). A correlation between HIF-2α and Ktrans p5th was found (r=0.30, P=0.02), but no differences in Ktrans, kep and MVD were observed for different levels of HIF expression. HIF expression was not associated with Gleason score. In conclusion, in this whole-mount prostate cancer study, larger prostate tumors showed frequently high HIF-2α expression, suggesting that larger tumors are clinically most relevant. However, HIF-1α and HIF-2α were not correlated with DCE-MRI parameters. Given the pronounced expression of HIF-2α and independence of Gleason score, HIF expression may function as a biomarker to guide boost dose prescription.

Original languageEnglish (US)
Pages (from-to)2249-2254
Number of pages6
JournalOncology Reports
Volume29
Issue number6
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

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Keywords

  • HIF-1α
  • HIF-2α
  • K
  • K
  • Prostate cancer

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Borren, A., Groenendaal, G., Van Der Groep, P., Moman, M. R., Boeken Kruger, A. E., Van Der Heide, U. A., ... Philippens, M. E. P. (2013). Expression of hypoxia-inducible factor-1α and -2α in whole-mount prostate histology: Relation with dynamic contrast-enhanced MRI and Gleason score. Oncology Reports, 29(6), 2249-2254. https://doi.org/10.3892/or.2013.2392