TY - JOUR
T1 - Expression of hypoxia-inducible factor-1α and -2α in whole-mount prostate histology
T2 - Relation with dynamic contrast-enhanced MRI and Gleason score
AU - Borren, Alie
AU - Groenendaal, Greetje
AU - Van Der Groep, Petra
AU - Moman, Maaike R.
AU - Boeken Kruger, Arto E.
AU - Van Der Heide, Uulke A.
AU - Jonges, Trudy N.
AU - Van Diest, Paul J.
AU - Van Vulpen, Marco
AU - Philippens, Marielle E.P.
PY - 2013/6
Y1 - 2013/6
N2 - The aim of this study was to investigate the association between the immunohistochemical expression of hypoxia-inducible factor (HIF)-1α and HIF-2α and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) parameters Ktrans and kep in prostate cancer. Therefore, 15 patients with biopsy-confirmed prostate cancer underwent a pre-operative 3T DCE-MRI scan. Immunohistochemical analysis of HIF-1α and HIF-2α, and of CD31 for microvessel density (MVD) was performed. Tumor areas were delineated on whole-mount histopathological sections. Nuclear HIF expression was correlated with the quantitative DCE-MRI parameters Ktrans and kep, MVD and Gleason score. HIF expression was highly heterogeneous within tumors and between patients. Pronounced expression of HIF-2α was present, while HIF-1α expression was more limited. Larger tumors showed higher HIF-2α expression (P=0.041). A correlation between HIF-2α and Ktrans p5th was found (r=0.30, P=0.02), but no differences in Ktrans, kep and MVD were observed for different levels of HIF expression. HIF expression was not associated with Gleason score. In conclusion, in this whole-mount prostate cancer study, larger prostate tumors showed frequently high HIF-2α expression, suggesting that larger tumors are clinically most relevant. However, HIF-1α and HIF-2α were not correlated with DCE-MRI parameters. Given the pronounced expression of HIF-2α and independence of Gleason score, HIF expression may function as a biomarker to guide boost dose prescription.
AB - The aim of this study was to investigate the association between the immunohistochemical expression of hypoxia-inducible factor (HIF)-1α and HIF-2α and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) parameters Ktrans and kep in prostate cancer. Therefore, 15 patients with biopsy-confirmed prostate cancer underwent a pre-operative 3T DCE-MRI scan. Immunohistochemical analysis of HIF-1α and HIF-2α, and of CD31 for microvessel density (MVD) was performed. Tumor areas were delineated on whole-mount histopathological sections. Nuclear HIF expression was correlated with the quantitative DCE-MRI parameters Ktrans and kep, MVD and Gleason score. HIF expression was highly heterogeneous within tumors and between patients. Pronounced expression of HIF-2α was present, while HIF-1α expression was more limited. Larger tumors showed higher HIF-2α expression (P=0.041). A correlation between HIF-2α and Ktrans p5th was found (r=0.30, P=0.02), but no differences in Ktrans, kep and MVD were observed for different levels of HIF expression. HIF expression was not associated with Gleason score. In conclusion, in this whole-mount prostate cancer study, larger prostate tumors showed frequently high HIF-2α expression, suggesting that larger tumors are clinically most relevant. However, HIF-1α and HIF-2α were not correlated with DCE-MRI parameters. Given the pronounced expression of HIF-2α and independence of Gleason score, HIF expression may function as a biomarker to guide boost dose prescription.
KW - HIF-1α
KW - HIF-2α
KW - K
KW - Prostate cancer
UR - http://www.scopus.com/inward/record.url?scp=84876209919&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876209919&partnerID=8YFLogxK
U2 - 10.3892/or.2013.2392
DO - 10.3892/or.2013.2392
M3 - Article
C2 - 23588703
AN - SCOPUS:84876209919
SN - 1021-335X
VL - 29
SP - 2249
EP - 2254
JO - Oncology reports
JF - Oncology reports
IS - 6
ER -