Expression of HLA-DR antigens on peripheral blood T lymphocytes and renal graft tubular epithelial cells in association with rejecion

Felix C. Henny, Jan J. Weening, William M. Baldwin, Paul J. Oljans, Hans J. Tanke, Leendert A. Van Es, Leendert C. Paul

Research output: Contribution to journalArticle

Abstract

Since the expression of HLA-DR antigens on peripheral blood T lymphocytes and renal graft tubular epithelial cells may be associated with immunological stimulation, we investigated the expession of these antigens on blood T lymphocytes and graft tubular epithelium in 84 renal transplant patients. Peripheral blood T lymphocytes were monitored by flow cytometry during the first 3 months after transplantation. Since the DR+ lymphocytes were selected by a double-labeling technique used for the Leu2a phenotype, the majority of the DR+ lymphocyts were also Leu2a+ with a small percentae of unidentified Leu2a- lymphocytes. Forty-one patients were treated with cyclosporine (CsA) and 43 with azathioprine (Aza), while both groups received low-dose steroids. Frozen sections of 57 renal biopsies from 43 patients (21 on CsA and 22 on Aza) were stained for HLA-DR antigens. In the Aza group, clinical rejection episoes correlated with an increased percentage of DR+ peripheral lymphocytes (P = 0.0005), and the expression of DR antigens on graft epithelial cells (P+ peripheral lymphocytes correlated with positive staining of the renal tubular cells for HLA-DR antigens (P

Original languageEnglish (US)
Pages (from-to)479-483
Number of pages5
JournalTransplantation
Volume42
Issue number5
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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    Henny, F. C., Weening, J. J., Baldwin, W. M., Oljans, P. J., Tanke, H. J., Van Es, L. A., & Paul, L. C. (1986). Expression of HLA-DR antigens on peripheral blood T lymphocytes and renal graft tubular epithelial cells in association with rejecion. Transplantation, 42(5), 479-483.