Expression of Histocompatibility-2 antigens on cultured cell lines derived from mouse blastocysts

Suzanne Ostrand-Rosenberg, Craig Hammerberg, Michael Edidin, Michael I. Sherman

Research output: Contribution to journalArticlepeer-review

Abstract

Four cell lines derived from four-day-old SWR/J♀×SJL/J♂ mouse blastocysts have been assayed for their expression of H-2 specificities with pauci- and monospecific H-2 typing sera. Direct microcytotoxicity and indirect absorption studies reveal many deviations from expected expression of particular H-2 specificities based on the cell lines' genotypes and on H-2 typing of adult F1 lymphocytes. No pattern of selective expression of public or private specificities of D-end or K- end specificities or of inclusion groups was noted. At least one public or private specificity of each Dq, Kq, Ds, and Ks region is present, indicating that part of each product is expressed. The partial expression of H-2 specificities is discussed structurally, in terms of how incomplete H-2 molecules may be present on the cell surface, and developmentally, in terms of how the variant H-2 specificities may be involved in cell positioning during ontogenesis.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalImmunogenetics
Volume4
Issue number1
DOIs
StatePublished - Dec 1 1977

ASJC Scopus subject areas

  • Immunology
  • Genetics

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