Expression of growth/differentiation factor 1 in the nervous system

Conservation of a bicistronic structure

Se Jin Lee

Research output: Contribution to journalArticle

Abstract

Growth/differentiation factor 1 (GDF-1) is a recently described member of the transforming growth factor β superfamily isolated from a day-8.5 mouse embryo cDNA library. Northern (RNA) analysis of embryonic mRNA detected two GDF-1 transcripts [1.4 kilobases (kb) and 3.0 kb in length] displaying distinct temporal patterns of expression. Only the 3.0-kb transcript was detected in adult tissues, where its expression was restricted almost exclusively to the central nervous system. Comparison of murine and human brain cDNA sequences corresponding to the 3.0-kb transcript revealed high conservation of two nonoverlapping open reading frames with poor conservation of the intervening spacer region and the putative 5′ and 3′ untranslated sequences. By immunohistochemical analysis, the protein encoded by the downstream open reading frame (GDF-1) was detected exclusively in the brain, spinal cord, and peripheral nerves in day-14.5 mouse embryos. The upstream open reading frame encodes a protein of unknown function containing multiple putative membrane-spanning domains. These findings raise the possibility that this mRNA may give rise to two different proteins.

Original languageEnglish (US)
Pages (from-to)4250-4254
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number10
StatePublished - May 15 1991
Externally publishedYes

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Growth Differentiation Factor 1
Nervous System
Open Reading Frames
Embryonic Structures
Spinal Nerves
Messenger RNA
Proteins
5' Untranslated Regions
Brain
Transforming Growth Factors
3' Untranslated Regions
Gene Library
Peripheral Nerves
Spinal Cord
Central Nervous System
Complementary DNA
RNA
Membranes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Growth/differentiation factor 1 (GDF-1) is a recently described member of the transforming growth factor β superfamily isolated from a day-8.5 mouse embryo cDNA library. Northern (RNA) analysis of embryonic mRNA detected two GDF-1 transcripts [1.4 kilobases (kb) and 3.0 kb in length] displaying distinct temporal patterns of expression. Only the 3.0-kb transcript was detected in adult tissues, where its expression was restricted almost exclusively to the central nervous system. Comparison of murine and human brain cDNA sequences corresponding to the 3.0-kb transcript revealed high conservation of two nonoverlapping open reading frames with poor conservation of the intervening spacer region and the putative 5′ and 3′ untranslated sequences. By immunohistochemical analysis, the protein encoded by the downstream open reading frame (GDF-1) was detected exclusively in the brain, spinal cord, and peripheral nerves in day-14.5 mouse embryos. The upstream open reading frame encodes a protein of unknown function containing multiple putative membrane-spanning domains. These findings raise the possibility that this mRNA may give rise to two different proteins.",
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N2 - Growth/differentiation factor 1 (GDF-1) is a recently described member of the transforming growth factor β superfamily isolated from a day-8.5 mouse embryo cDNA library. Northern (RNA) analysis of embryonic mRNA detected two GDF-1 transcripts [1.4 kilobases (kb) and 3.0 kb in length] displaying distinct temporal patterns of expression. Only the 3.0-kb transcript was detected in adult tissues, where its expression was restricted almost exclusively to the central nervous system. Comparison of murine and human brain cDNA sequences corresponding to the 3.0-kb transcript revealed high conservation of two nonoverlapping open reading frames with poor conservation of the intervening spacer region and the putative 5′ and 3′ untranslated sequences. By immunohistochemical analysis, the protein encoded by the downstream open reading frame (GDF-1) was detected exclusively in the brain, spinal cord, and peripheral nerves in day-14.5 mouse embryos. The upstream open reading frame encodes a protein of unknown function containing multiple putative membrane-spanning domains. These findings raise the possibility that this mRNA may give rise to two different proteins.

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