Expression of glutathione S-transferase-cardiotoxin fusion protein in Escherichia coli

Lang Ming Chi, Alka A. Vyas, Gordon S. Rule, Wen guey Wu

Research output: Contribution to journalArticle

Abstract

We report here the construction of cardiotoxin V gene, from cobra snake venom (Naja naja atra), by chemically synthesized oligonucleotides and its expression as a glutathione S-transferase-cardiotoxin fusion protein in the inclusion bodies of Escherichia coli. The expression of cardiotoxin fusion protein with a yield of about 35 mg/liter culture was confirmed by highly specific anti-peptide antibodies generated against the unique amino acid residues located at the tip of loop II of cardiotoxin V. Since the fusion protein can be easily treated by CNBr to free the toxin moiety, as revealed by immunoblotting of the cleaved protein, the results provide an avenue for future structural and functional studies of cardiotoxin molecules.

Original languageEnglish (US)
Pages (from-to)1679-1683
Number of pages5
JournalToxicon
Volume32
Issue number12
DOIs
StatePublished - 1994
Externally publishedYes

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Cardiotoxins
Escherichia coli Proteins
Glutathione Transferase
Escherichia coli
Fusion reactions
Proteins
Cobra Venoms
Elapidae
Snake Venoms
Inclusion Bodies
Immunoblotting
Oligonucleotides
Anti-Idiotypic Antibodies
Genes
Amino Acids
Peptides
Molecules
Antibodies

ASJC Scopus subject areas

  • Toxicology

Cite this

Expression of glutathione S-transferase-cardiotoxin fusion protein in Escherichia coli. / Chi, Lang Ming; Vyas, Alka A.; Rule, Gordon S.; Wu, Wen guey.

In: Toxicon, Vol. 32, No. 12, 1994, p. 1679-1683.

Research output: Contribution to journalArticle

Chi, Lang Ming ; Vyas, Alka A. ; Rule, Gordon S. ; Wu, Wen guey. / Expression of glutathione S-transferase-cardiotoxin fusion protein in Escherichia coli. In: Toxicon. 1994 ; Vol. 32, No. 12. pp. 1679-1683.
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