Expression of genes for B7-H3 and other T cell ligands by nasal epithelial cells during differentiation and activation

Bahman Saatian, Xiao Ying Yu, Andrew P. Lane, Thanh Doyle, Vincenzo Casolaro, Ernst Wm Spannhake

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Epithelial cells of the human respiratory tract express human leukocyte antigen (HLA) and the costimulatory molecules B7-1 and B7-2. Little is known, however, about the constitutive expression of genes encoding for the more recently identified members of the B7 homolog family of costimulatory molecules or about the influence of cellular differentiation and cytokines on their activity or on that of HLA or B7-1 and B7-2. Human nasal epithelial (HNE) cells were grown at the air-liquid interface (ALI) for 2 or 21 days to model in vivo conditions. Expression of genes for HLA-B and HLA-DR1 increased during mucociliary differentiation during this period and became more similar to HNE cells obtained fresh by brush biopsy from nasal turbinates. Gene transcripts for B7-H3 and B7-H2 were abundantly expressed in cells cultured at the ALI, but neither their activities nor that of B7-2 was significantly altered during differentiation. IFN-γ and TNF-α upregulated mRNA encoding for both HLA molecules, but not for the B7 molecules. This study describes, for the first time, the expression of B7-H3 and B7-H2 by HNE cells and thus expands the range of potential costimulatory signals through which these cells may interact with activated mucosal T lymphocytes. In addition, the results suggest that the extent of mucociliary differentiation of cultured cells may influence this capability.

Original languageEnglish (US)
Pages (from-to)L217-L225
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume287
Issue number1 31-1
DOIs
StatePublished - Jul 2004

Keywords

  • Air-liquid interface
  • Human
  • Interferon-γ
  • Primary cell culture
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Fingerprint

Dive into the research topics of 'Expression of genes for B7-H3 and other T cell ligands by nasal epithelial cells during differentiation and activation'. Together they form a unique fingerprint.

Cite this