Expression of functional interleukin 2 receptors by peripheral blood monocytes from patients with active pulmonary tuberculosis

Zahra Toossi, John R. Sedor, John P. Lapurga, Robert J. Ondash, Jerrold J. Ellner

Research output: Contribution to journalArticle

Abstract

Peripheral blood monocytes from patients with active tuberculosis are "activated" by a number of criteria, including selective depression of T-lymphocyte responses to the mycobacterial antigen, tuberculin-purified protein derivative (PPD). We studied monocytes from patients with tuberculosis and healthy skin test reactive controls for expression and function of IL 2 receptors (IL 2R). Depletion of adherent monocytes increased the IL 2 activity of supernatants of PPD-stimulated T cell cultures from patients by 30-fold. When cultured with purified IL 2, adherent cells from the patients depleted IL 2 activity by 66%. Monocytes from patients displayed IL 2R on their surface as evidenced by anti-Tac reactivity, and released soluble IL 2R into the medium during culture. The release of soluble IL 2R was augmented when monocytes were cultured with PPD. Finally, freshly isolated adherent monocytes from patients but not healthy individuals expressed the gene encoding Tac protein. Thus, blood monocytes from patients with tuberculosis express functional IL 2R constitutively. This property may be important in the immunoregulatory and effector function of mononuclear phagocytes during tuberculosis.

Original languageEnglish (US)
Pages (from-to)1777-1784
Number of pages8
JournalJournal of Clinical Investigation
Volume85
Issue number6
StatePublished - Jun 1990
Externally publishedYes

Keywords

  • IL 2R
  • Monocyte
  • Tuberculosis

ASJC Scopus subject areas

  • Medicine(all)

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