Expression of fetal isoforms of actin after transplantation injury

Renkui Xu, James F. Burdick, William Beschorner, Chumpon Wilasrusmee, Dilip S. Kittur

Research output: Contribution to journalArticlepeer-review

Abstract

Trauma and injury to transplanted organs in the early post-transplant period are significant factors that affect long-term graft survival. Fetal isoforms of actin are integral members of the immediate early gene family and are expressed in response to free radical injury. We therefore studied actin gene expression in heart transplantation to determine if reperfusion injury activates fetal isoforms of actins. Heterotopic cardiac transplantations were performed in mice. mRNA was extracted from allo- and isografted hearts as well as from normal hearts and spleen. Northern hybridization with actin cDNA to α and β/γ actin mRNA was performed and analyzed by densitometry. The β/γ actin gene expression in the transplanted hearts was found to be significantly elevated within 48 h after transplantation. Analysis of β/γ actin gene expression in isografts substantiates the possibility of de novo increase in actin expression. Our studies demonstrate for the first time that fetal isoforms of actin are induced in the allograft heart after transplantation.

Original languageEnglish (US)
Pages (from-to)296-301
Number of pages6
JournalTransplant International
Volume15
Issue number6
DOIs
StatePublished - 2002

Keywords

  • Actin gene expression
  • Early immediate genes
  • Gene express ion
  • MHC
  • Mouse heart grafts

ASJC Scopus subject areas

  • Transplantation

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