Expression of fatty acid synthase depends on NAC1 and is associated with recurrent ovarian serous carcinomas

Ie Ming Shih, Stefanie M. Ueda, Kai Lee Yap, Ben Davidson, Yuan Tian, Vivek Murthy, Tian-Li Wang, Kala Visvanathan, Francis P. Kuhajda, Robert E. Bristow, Hui Zhang

Research output: Contribution to journalArticle

Abstract

Our previous reports demonstrated that NAC1, a BTB/POZ domain-containing nuclear protein, upregulates in recurrent ovarian serous carcinoma and participates in developing drug resistance in cancer cells. The current study applies quantitative proteomics to identify the proteins controlled by NAC1 by comparing the proteomes of SKOV3 cells with and without expression of a dominant negative NAC1 construct, N130. From the proteins that are downregulated by N130 (upregulated by NAC1), we chose to further characterize fatty acid synthase (FASN). Similar to change in protein level, the FASN transcript level in SKOV3 cells was significantly reduced by N130 induction or by NAC1 knockdown. Immunohistochemistry showed that NAC1 and FASN immunointensities in ovarian serous carcinoma tissues had a highly significant correlation (P

Original languageEnglish (US)
Article number285191
JournalJournal of Oncology
DOIs
StatePublished - 2010

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Fatty Acid Synthases
Carcinoma
Proteins
Proteome
Nuclear Proteins
Drug Resistance
Proteomics
Up-Regulation
Down-Regulation
Immunohistochemistry
Neoplasms

ASJC Scopus subject areas

  • Oncology

Cite this

Expression of fatty acid synthase depends on NAC1 and is associated with recurrent ovarian serous carcinomas. / Shih, Ie Ming; Ueda, Stefanie M.; Yap, Kai Lee; Davidson, Ben; Tian, Yuan; Murthy, Vivek; Wang, Tian-Li; Visvanathan, Kala; Kuhajda, Francis P.; Bristow, Robert E.; Zhang, Hui.

In: Journal of Oncology, 2010.

Research output: Contribution to journalArticle

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AU - Shih, Ie Ming

AU - Ueda, Stefanie M.

AU - Yap, Kai Lee

AU - Davidson, Ben

AU - Tian, Yuan

AU - Murthy, Vivek

AU - Wang, Tian-Li

AU - Visvanathan, Kala

AU - Kuhajda, Francis P.

AU - Bristow, Robert E.

AU - Zhang, Hui

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