Expression of c-raf-1 and A-raf-1 during regeneration of rat liver following surgical partial hepatectomy

J. A. Silverman, J. Zurlo, M. A. Watson, James D Yager

Research output: Contribution to journalArticle

Abstract

The major objective of this study was to investigate the expression of members of the raf family of proto-oncogenes during rat liver regeneration. The steady-state level of expression of both c-raf-1 and A-raf-1 increased three- to fivefold 18-24 h following partial hepatectomy, and it returned to basal levels by 72 h. Expression of c-myc and Ha-ras mRNA was increased at 3 and 18-24 h, respectively, confirming previous reports. Increased steady-state levels of c-raf-1, A-raf-1, and Ha-ras mRNA were also detected in heptatocytes isolated from rat liver 24 h after partial hepatectomy. Thus, elevated expression of the raf genes closely correlated with that of Ha-ras, beginning at 12 h and reaching maximal levels during the first peak of DNA synthesis following partial hepatectomy.

Original languageEnglish (US)
Pages (from-to)63-67
Number of pages5
JournalMolecular Carcinogenesis
Volume2
Issue number2
StatePublished - 1989
Externally publishedYes

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Liver Regeneration
Hepatectomy
Messenger RNA
Proto-Oncogenes
Gene Expression
Liver
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Expression of c-raf-1 and A-raf-1 during regeneration of rat liver following surgical partial hepatectomy. / Silverman, J. A.; Zurlo, J.; Watson, M. A.; Yager, James D.

In: Molecular Carcinogenesis, Vol. 2, No. 2, 1989, p. 63-67.

Research output: Contribution to journalArticle

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