Expression of c-raf-1 and A-raf-1 during differentiation of 3T3-L1 preadipocyte fibroblasts into adipocytes

Antanina Zmuidzinas, Gwyn W. Gould, James D. Yager

Research output: Contribution to journalArticle

Abstract

The objective of this study was to examine the expression of the c-raf-1 and A-raf-1 protooncogenes during differentiation of 3T3-L1 preadipocytes into adipocytes. At confluence, prior to initiation of differentiation c-raf and A-raf steady state mRNA levels were low. Expression of c-raf and A-raf began to increase 72 hours following initiation of differentiation by treatment with differentiation medium, reaching a maximum increase of 3 to 6-fold and 3 to 4-fold respectively by 190 hours. The increase of c-raf and A-raf steady state message levels occurred concomittent with the onset of differentiation as indicated by increased levels of glycerol-3-phosphate dehydrogenase mRNA. These changes were compared with those for several other protooncogene mRNAs including c-myc, c-fos, H-ras and histone H3. These results are the first to show increased expression of the raf protooncogenes during terminal differentiation rather than in association with proliferation.

Original languageEnglish (US)
Pages (from-to)1180-1187
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume162
Issue number3
DOIs
StatePublished - Aug 15 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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