TY - JOUR
T1 - Expression of c-raf-1 and A-raf-1 during differentiation of 3T3-L1 preadipocyte fibroblasts into adipocytes
AU - Zmuidzinas, Antanina
AU - Gould, Gwyn W.
AU - Yager, James D.
N1 - Funding Information:
We thank Dr. Susan Brown for maintaining the cell cultures. We are grateful to Dr. T. Sugimura, Dr. R. Ellis, Dr. J. Stein, Dr. B.M. Paterson, Dr. M. Mueckler and Dr. B.M. Spiegelman for providing the plasmids described in materials and methods. This work was supported by USPHS NC1 grant CA 36713 (to JDY), Cancer Center Core Grant CA 23108 to the Norris Cotton Cancer Center, Juvenile Diabetes Foundation International Fellowship (to G.W.G), and by NIH grant DK 25336 (to Gustav E. Lienhard).
PY - 1989/8/15
Y1 - 1989/8/15
N2 - The objective of this study was to examine the expression of the c-raf-1 and A-raf-1 protooncogenes during differentiation of 3T3-L1 preadipocytes into adipocytes. At confluence, prior to initiation of differentiation c-raf and A-raf steady state mRNA levels were low. Expression of c-raf and A-raf began to increase 72 hours following initiation of differentiation by treatment with differentiation medium, reaching a maximum increase of 3 to 6-fold and 3 to 4-fold respectively by 190 hours. The increase of c-raf and A-raf steady state message levels occurred concomittent with the onset of differentiation as indicated by increased levels of glycerol-3-phosphate dehydrogenase mRNA. These changes were compared with those for several other protooncogene mRNAs including c-myc, c-fos, H-ras and histone H3. These results are the first to show increased expression of the raf protooncogenes during terminal differentiation rather than in association with proliferation.
AB - The objective of this study was to examine the expression of the c-raf-1 and A-raf-1 protooncogenes during differentiation of 3T3-L1 preadipocytes into adipocytes. At confluence, prior to initiation of differentiation c-raf and A-raf steady state mRNA levels were low. Expression of c-raf and A-raf began to increase 72 hours following initiation of differentiation by treatment with differentiation medium, reaching a maximum increase of 3 to 6-fold and 3 to 4-fold respectively by 190 hours. The increase of c-raf and A-raf steady state message levels occurred concomittent with the onset of differentiation as indicated by increased levels of glycerol-3-phosphate dehydrogenase mRNA. These changes were compared with those for several other protooncogene mRNAs including c-myc, c-fos, H-ras and histone H3. These results are the first to show increased expression of the raf protooncogenes during terminal differentiation rather than in association with proliferation.
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U2 - 10.1016/0006-291X(89)90798-5
DO - 10.1016/0006-291X(89)90798-5
M3 - Article
C2 - 2669746
AN - SCOPUS:0024363752
SN - 0006-291X
VL - 162
SP - 1180
EP - 1187
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -