Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina

Zhi Ping Mi, Peihua Jiang, Weili Weng, Frederik P. Lindberg, Vinodh Narayanan, Carl F. Lagenaur

Research output: Contribution to journalArticle

Abstract

P84 and integrin associated protein (IAP) are heterophilic binding partners that are expressed in the central nervous system in addition to a variety of other tissues. Both molecules are known to be involved in cell signaling in nonneural tissues. In the retina, both molecules are expressed prominently in plexiform layers, suggesting a possible association with synapses. Here, we examined the cellular expression and ultrastructural localization of the two molecules in the developing mouse retina. Both appeared to be expressed at one or both sides of synaptic sites, although the expression of IAP in the retina precedes that of P84. Examination of transgenic IAP-null retinae revealed a failure of P84 to become associated with synaptic sites, suggesting the interaction of P84 with IAP was necessary for P84's synaptic localization. These findings suggest that the signaling activities of P84 and IAP are localized to sites of synaptic contact in the retina. Thus this pair of synapse-associated molecules represents a bidirectional signaling system that could function to modify synaptic activity or possibly trophic interactions between central neurons.

Original languageEnglish (US)
Pages (from-to)335-344
Number of pages10
JournalJournal of Comparative Neurology
Volume416
Issue number3
DOIs
StatePublished - Jan 17 2000
Externally publishedYes

Fingerprint

Integrins
Synapses
Retina
Membrane Proteins
Ligands
Proteins
Protein Binding
Central Nervous System
Mouse Cd47 protein
Neurons

Keywords

  • CD47
  • IAP
  • P84
  • Ribbon synapse
  • SHPS-1
  • SIRP

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina. / Mi, Zhi Ping; Jiang, Peihua; Weng, Weili; Lindberg, Frederik P.; Narayanan, Vinodh; Lagenaur, Carl F.

In: Journal of Comparative Neurology, Vol. 416, No. 3, 17.01.2000, p. 335-344.

Research output: Contribution to journalArticle

Mi, Zhi Ping ; Jiang, Peihua ; Weng, Weili ; Lindberg, Frederik P. ; Narayanan, Vinodh ; Lagenaur, Carl F. / Expression of a synapse-associated membrane protein, P84/SHPS-1, and its ligand, IAP/CD47, in mouse retina. In: Journal of Comparative Neurology. 2000 ; Vol. 416, No. 3. pp. 335-344.
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