Expression of a smaller lecithin:retinol acyl transferase transcript and reduced retinol esterification in MCF-7 cells

Fausto Andreola, Valeria Giandomenico, Richard Spero, Luigi M. De Luca

Research output: Contribution to journalArticle

Abstract

Retinyl ester concentration is regulated by retinoic acid (RA) through an autoregulatory loop, which acts on lecithin:retinol acyltransferase (LRAT). We tested whether retinol esterification activity is downregulated in human mammary carcinoma ceils and whether LRAT expression is RAR-regulated. Normal human mammary epithelial (HMEC) cells expressed a retinoid-upregulated 5-kb LRAT transcript and synthesized retinyl esters from 3H-retinol. Human carcinoma MCF-7 cells failed to express the 5-kb LRAT transcript and to synthesize retinyl esters. Instead, they expressed a 2.7-kb LRAT transcript. Both transcripts were upregulated by RA. Stable expression of the dominant-negative RARα403 blunted the up-regulation of LRAT mRNA by RA. We conclude that retinol esterification is decreased in MCF-7 vs normal mammary cells; that these cancer cells express a shorter (2.7 kb) LRAT transcript, and that retinoid receptors are involved in the regulation of LRAT-mediated retinyl ester synthesis by RA.

Original languageEnglish (US)
Pages (from-to)920-924
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume279
Issue number3
DOIs
StatePublished - Dec 29 2000
Externally publishedYes

Keywords

  • Breast cancer
  • LRAT
  • RAR
  • Retinyl esters

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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