Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging

Hong Jun Song, Guo Li Ming, Edward Fon, Elizabeth Bellocchio, Robert H. Edwards, Mu Ming Poo

Research output: Contribution to journalArticle

Abstract

A putative vesicular acetylcholine transporter (VAChT) was overexpressed in developing Xenopus spinal neurons by injection of rat VAChT cDNA or synthetic mRNA into Xenopus embryos. This resulted in a marked increase in the amplitude and frequency of miniature excitatory postsynaptic currents at neuromuscular synapses, reflecting an over 10-fold increase in the vesicular packaging of acetylcholine (ACh). The effect appeared in developing neurons even before synaptogenesis and was blocked by L-vesamicol, a specific blocker of ACh uptake into synaptic vesicles. Mutational studies showed that two highly conserved aspartate residues within putative transmembrane domains 4 and 10 are essential for the transport activity. These results provide direct evidence for the physiological function of a putative VAChT and demonstrate that quantal size can be regulated by changes in vesicular transporter activity.

Original languageEnglish (US)
Pages (from-to)815-826
Number of pages12
JournalNeuron
Volume18
Issue number5
DOIs
StatePublished - May 1997
Externally publishedYes

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Vesicular Acetylcholine Transport Proteins
Product Packaging
Xenopus
Acetylcholine
Neurons
Spinal Injections
Synaptic Vesicles
Excitatory Postsynaptic Potentials
Aspartic Acid
Synapses
Embryonic Structures
Complementary DNA
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Song, H. J., Ming, G. L., Fon, E., Bellocchio, E., Edwards, R. H., & Poo, M. M. (1997). Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging. Neuron, 18(5), 815-826. https://doi.org/10.1016/S0896-6273(00)80320-7

Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging. / Song, Hong Jun; Ming, Guo Li; Fon, Edward; Bellocchio, Elizabeth; Edwards, Robert H.; Poo, Mu Ming.

In: Neuron, Vol. 18, No. 5, 05.1997, p. 815-826.

Research output: Contribution to journalArticle

Song, HJ, Ming, GL, Fon, E, Bellocchio, E, Edwards, RH & Poo, MM 1997, 'Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging', Neuron, vol. 18, no. 5, pp. 815-826. https://doi.org/10.1016/S0896-6273(00)80320-7
Song, Hong Jun ; Ming, Guo Li ; Fon, Edward ; Bellocchio, Elizabeth ; Edwards, Robert H. ; Poo, Mu Ming. / Expression of a putative vesicular acetylcholine transporter facilitates quantal transmitter packaging. In: Neuron. 1997 ; Vol. 18, No. 5. pp. 815-826.
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