Expression of a novel nuclear protein in activated and in tat-I expressing T cells

Anna Bielinska, Leslie Baier, Nabil Hailat, John R. Strahler, Gary J. Nabel, Sam Hanash

Research output: Contribution to journalArticle

Abstract

The intracellular events that occur in T lymphoid cells after activation or after infection with HIV-1 are not well defined. In the case of HIV-1 infection, it is unknown whether the tat-I gene, an essential gene for viral replication, affects host cell nuclear factors. Using two-dimensional PAGE, we have identified a novel nuclear protein, designated nuclear protein-28,000 (NP-28), which is induced in Jurkat T cells by stimulation with PMA and/or PHA or ionomycin. This nuclear protein has an apparent molecular mass of 28,000 Da and an isoelectric point of 4.6. Interestingly, Jurkat cells transfected with tat-I express higher levels of NP-28 constitutively, without added stimulation. Incubation of Jurkat cells expressing tat-I with PMA and/or PHA or ionomycin causes superinduction of NP-28. We have therefore identified a novel lymphoid nuclear protein induced by T cell activation that occurs in tat-I expressing cells in the absence of activating agents.

Original languageEnglish (US)
Pages (from-to)1031-1036
Number of pages6
JournalJournal of Immunology
Volume146
Issue number3
StatePublished - Feb 1 1991
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Bielinska, A., Baier, L., Hailat, N., Strahler, J. R., Nabel, G. J., & Hanash, S. (1991). Expression of a novel nuclear protein in activated and in tat-I expressing T cells. Journal of Immunology, 146(3), 1031-1036.