Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

F. G. Rodrigues, M. N. Santos, T. X.T. De Carvalho, B. C. Rocha, M. A. Riehle, P. F.P. Pimenta, E. G. Abraham, M. Jacobs-Lorena, C. F. Alves De Brito, L. A. Moreira

Research output: Contribution to journalArticlepeer-review

Abstract

The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalInsect molecular biology
Volume17
Issue number2
DOIs
StatePublished - Apr 2008

Keywords

  • Bee venom phospholipase A
  • Fitness
  • Malaria
  • Transformation
  • piggyBac

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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