Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

F. G. Rodrigues; M. N. Santos; T. X.T. De Carvalho; B. C. Rocha; M. A. Riehle; P. F.P. Pimenta; E. G. Abraham; M. Jacobs-Lorena; C. F. Alves De Brito; L. A. Moreira

doi:10.1111/j.1365-2583.2008.00791.x

Expression of a mutated phospholipase A₂ in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development

F. G. Rodrigues, M. N. Santos, T. X.T. De Carvalho, B. C. Rocha, M. A. Riehle, P. F.P. Pimenta, E. G. Abraham, M. Jacobs-Lorena, C. F. Alves De Brito, L. A. Moreira

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A₂ (PLA₂) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA₂ blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA₂ (mPLA₂) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA₂.

Original language	English (US)
Pages (from-to)	175-183
Number of pages	9
Journal	Insect molecular biology
Volume	17
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2583.2008.00791.x
State	Published - Apr 2008

Keywords

Bee venom phospholipase A
Fitness
Malaria
Transformation
piggyBac

ASJC Scopus subject areas

Molecular Biology
Genetics
Insect Science

Access to Document

10.1111/j.1365-2583.2008.00791.x

Cite this

Rodrigues, F. G., Santos, M. N., De Carvalho, T. X. T., Rocha, B. C., Riehle, M. A., Pimenta, P. F. P., Abraham, E. G., Jacobs-Lorena, M., Alves De Brito, C. F., & Moreira, L. A. (2008). Expression of a mutated phospholipase A₂ in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development. Insect molecular biology, 17(2), 175-183. https://doi.org/10.1111/j.1365-2583.2008.00791.x

Rodrigues, FG, Santos, MN, De Carvalho, TXT, Rocha, BC, Riehle, MA, Pimenta, PFP, Abraham, EG, Jacobs-Lorena, M, Alves De Brito, CF & Moreira, LA 2008, 'Expression of a mutated phospholipase A₂ in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development', Insect molecular biology, vol. 17, no. 2, pp. 175-183. https://doi.org/10.1111/j.1365-2583.2008.00791.x

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abstract = "The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.",

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