cDNA corresponding to human IgE heavy (ε) chain mRNA was cloned from human IgE-secreting myeloma U255 cells. Partial nucleotide sequence analysis demonstrated that the cloned cDNA contained the coding region for about two-thirds of the C(H)2 and all of the C(H)3 and C(H)4 domains as well as the 3'-untranslated region. This ε DNA was inserted into expression vector pUC7 and expression of an ε-chain fragment in Escherichia coli was demonstrated by protein blot analysis using 125I-labeled anti-human IgE as probe. The expression product was purified on a column of goat anti-human IgE-conjugated Sepharose 4B and the polypeptide was found to retain binding activity to human basophils.
|Original language||English (US)|
|Number of pages||5|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|Issue number||17 I|
|State||Published - Jan 1 1984|
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