Expression of a biologically active fragment of human IgE ε chain in Escherichia coli

Liu Fu-Tong Liu, K. A. Albrandt, C. G. Bry, T. Ishizaka

Research output: Contribution to journalArticle

Abstract

cDNA corresponding to human IgE heavy (ε) chain mRNA was cloned from human IgE-secreting myeloma U255 cells. Partial nucleotide sequence analysis demonstrated that the cloned cDNA contained the coding region for about two-thirds of the C(H)2 and all of the C(H)3 and C(H)4 domains as well as the 3'-untranslated region. This ε DNA was inserted into expression vector pUC7 and expression of an ε-chain fragment in Escherichia coli was demonstrated by protein blot analysis using 125I-labeled anti-human IgE as probe. The expression product was purified on a column of goat anti-human IgE-conjugated Sepharose 4B and the polypeptide was found to retain binding activity to human basophils.

Original languageEnglish (US)
Pages (from-to)5369-5373
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number17 I
StatePublished - Jan 1 1984

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