Expression and the role of cathepsin H in human glioma progression and invasion

Marupudi Sivaparvathi, Raymond Sawaya, Ziya L. Gokaslan, Kumar S. Chintala, Jasti S. Rao

Research output: Contribution to journalArticlepeer-review

Abstract

Proteinases and their inhibitors may play a role in the development and progression of many cancers. Several studies suggested that lysosomal proteinases cathepsin B, L, and D may be involved in the malignant progression of some human neoplastic diseases. In this study, we determined the levels of cathepsin H in human glioma progression and the significance of cathepsin H in glioma cell invasion. Levels of cathepsin H antigen were found to be significantly higher in glioblastomas and anaplastic astrocytoma when compared with normal brain tissue and low-grade gliomas. Western blotting confirmed the presence of authentic cathepsin H with a doublet at 27 and 25 kDa in normal brain tissue and tumor samples. However, the intensity of the band increased significantly in glioblastoma samples. Cathepsin H antibody inhibited the invasion of glioblastoma cell lines through Matrigel invasion assay. These data suggest that the tumor-specific increase in antigen may be a useful independent marker of tumor progression in central nervous system neoplasms.

Original languageEnglish (US)
Pages (from-to)121-126
Number of pages6
JournalCancer Letters
Volume104
Issue number1
DOIs
StatePublished - Jun 24 1996

Keywords

  • Cysteine proteases
  • Extracellular matrix
  • Glioblastoma multiform
  • Invasion

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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