Expression and regulation of CCR1 by airway smooth muscle cells in asthma

Philippe Joubert, Stephane Lajoie, Mélanie Welman, Stephane Dragon, Séverine Létuvée, Barbara Tolloczko, Andrew J. Halayko, Abdelilah Soussi Gounni, Karim Maghni, Qutayba Hamid

Research output: Contribution to journalArticle

Abstract

C-C chemokines such as CCL11, CCL5, and CCL3 are central mediators in the pathogenesis of asthma. They are mainly associated with the recruitment and the activation of specific inflammatory cells, such as eosinophils, lymphocytes, and neutrophils. It has recently been shown that they can also activate structural cells, such as airway smooth muscle and epithelial cells. The aims of this study were to examine the expression of the CCL3 receptor, CCR1, on human airway smooth muscle cells (ASMC) and to document the regulation of this receptor by cytokines involved in asthma pathogenesis. We first demonstrated that CCR1 mRNA is increased in the airways of asthmatic vs control subjects and showed for the first time that ASMC express CCR1 mRNA and protein, both in vitro and in vivo. Calcium mobilization by CCR1 ligands confirmed its functionality on ASMC. Stimulation of ASMC with TNF-α and, to a lesser extent, IFN-γ resulted in an up-regulation of CCR1 expression, which was totally suppressed by both dexamethasone or mithramycin. Taken together, our data suggest that CCR1 might be involved in the pathogenesis of asthma, through the activation of ASMC by its ligands.

Original languageEnglish (US)
Pages (from-to)1268-1275
Number of pages8
JournalJournal of Immunology
Volume180
Issue number2
DOIs
StatePublished - Jan 15 2008
Externally publishedYes

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Smooth Muscle Myocytes
Asthma
CCR1 Receptors
Plicamycin
Ligands
CC Chemokines
Messenger RNA
Cytokine Receptors
Eosinophils
Dexamethasone
Neutrophils
Up-Regulation
Epithelial Cells
Lymphocytes
Calcium
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Joubert, P., Lajoie, S., Welman, M., Dragon, S., Létuvée, S., Tolloczko, B., ... Hamid, Q. (2008). Expression and regulation of CCR1 by airway smooth muscle cells in asthma. Journal of Immunology, 180(2), 1268-1275. https://doi.org/10.4049/jimmunol.180.2.1268

Expression and regulation of CCR1 by airway smooth muscle cells in asthma. / Joubert, Philippe; Lajoie, Stephane; Welman, Mélanie; Dragon, Stephane; Létuvée, Séverine; Tolloczko, Barbara; Halayko, Andrew J.; Gounni, Abdelilah Soussi; Maghni, Karim; Hamid, Qutayba.

In: Journal of Immunology, Vol. 180, No. 2, 15.01.2008, p. 1268-1275.

Research output: Contribution to journalArticle

Joubert, P, Lajoie, S, Welman, M, Dragon, S, Létuvée, S, Tolloczko, B, Halayko, AJ, Gounni, AS, Maghni, K & Hamid, Q 2008, 'Expression and regulation of CCR1 by airway smooth muscle cells in asthma', Journal of Immunology, vol. 180, no. 2, pp. 1268-1275. https://doi.org/10.4049/jimmunol.180.2.1268
Joubert, Philippe ; Lajoie, Stephane ; Welman, Mélanie ; Dragon, Stephane ; Létuvée, Séverine ; Tolloczko, Barbara ; Halayko, Andrew J. ; Gounni, Abdelilah Soussi ; Maghni, Karim ; Hamid, Qutayba. / Expression and regulation of CCR1 by airway smooth muscle cells in asthma. In: Journal of Immunology. 2008 ; Vol. 180, No. 2. pp. 1268-1275.
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